Sample Preparation
Horizon Food & Environmental Products
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Peptide Chemistry
Flash Purification
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Industrial Products
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Service & Support
- Automated Sample Preparation Instruments
- Manual Sample Preparation & Accessories
- ISOLUTE® FILTER+ High Performance Filtration Plates
- ISOLUTE® PPT+ Protein Precipitation Plates
- ISOLUTE® PLD+ Protein and Phospholipid Removal Products
- ISOLUTE® HYDRO DME+ Dual Mode Extraction Products
- ISOLUTE® QuEChERS
- ISOLUTE® SLE+ Supported Liquid Extraction Products
- ISOLUTE® SPE Columns and Plates
-
- ISOLUTE® 101
- ISOLUTE® AL-A, AL-N & AL-B
- ISOLUTE® Accessories
- ISOLUTE® C18
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- ISOLUTE® ENV+ On-line SPE Cartridges
- ISOLUTE® EPH
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- ISOLUTE® TPH
- EVOLUTE® SPE Columns and Plates
- Biotage® Selekt
- ACI™ and Isolera™ Flash Systems
- Flash Columns
-
- Biotage® Sfär - Overview
- Biotage® Sfär Silica HC D
- Biotage® Sfär Silica D
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- Biotage® Sfär KP-Amino D
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- Biotage® SNAP Ultra
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- Biotage® SNAP Ultra C18
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- Biotage® SNAP - Samplet®
- Flash 75/150/400 Cartridges
- Flash Accessories
- Flash 75/150/400 Systems
- Service Agreements - Europe
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- Discontinued Products
-
- Biotage® SNAP Bio
- AFFINILUTE™ MIP Columns
- Biotage ZIP®
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- Biotage® Horizon SPE-Z
- Biotage® Initiator Classic
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- Biotage® V-10 Evaporator
- EVOLUTE® ABN
- EVOLUTE® AX
- EVOLUTE® CX
- EVOLUTE® WAX
- EVOLUTE® WCX
- EcoLine Environmental Filters
- FlashMaster Personal Plus
- KILOPREP Systems and Consumables
- RapidTrace®+
- SAM
- SAP
- SpeedVap® III
- TurboVap® 96
- TurboVap® Classic 500
- TurboVap® Classic II
- TurboVap® Classic LV
414169SP
Column Rack 24 x 1 mL, Biotage® Extrahera
Read more on the product page
» APPLICATION NOTES
Extraction of THC and its Metabolites from Human Hair Using ISOLUTE® SLE+ Prior to UPLC-MS/MS Analysis
This application note describes a procedure for sample pre-treatment and extraction of THC and metabolites from human hair, using Biotage® Lysera for matrix pulverization of the sample prior to clean up using ISOLUTE® SLE+ supported liquid extraction.
Manual processing protocols were developed using the Biotage® PRESSURE+ 96 (plate format) or 48 (column format) positive pressure manifolds. For automated processing, protocols were developed using Biotage® ExtraheraTM.
Tags:
11-hydroxy- Δ9-tetrahydrocannabinol (THC-OH),
11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH),
96-well plate,
Application Notes,
Bead Ruptor,
Cannabidiol,
Cannabinol,
Column,
English,
Extrahera,
Forensic,
Hair,
LC-MS/MS,
Lysera,
THC,
THC metabolites,
THC-COOH,
Δ9-tetrahydrocannabinol (THC)
Automated Extraction of Opiates from Hydrolyzed and Non-Hydrolyzed Urine Using ISOLUTE® SLE+ Prior to GC/MS Analysis
This application note describes the fully-automated extraction of opiates from acid-hydrolyzed urine and non-hydrolyzed urine prior to GC/MS analysis. The methods were automated using Biotage® Extrahera™ configured for use with ISOLUTE® SLE+ supported liquid extraction columns.
Using the Biotage® Extrahera™, 24 hydrolyzed samples were extracted in under 31 minutes and 24 non-hydrolyzed samples were extracted in under 36 minutes. The limits of quantitation meet or exceed the sensitivity requirements set by SAMHSA and EWDTS for workplace testing applications.
Tags:
6-acetylmorphine (MAM),
96-well plate,
Application Notes,
Codeine,
Dihydrocodeine (DHC),
English,
Extrahera,
Forensic,
GC-MS,
Hydrocodone,
Hydrolysed urine,
Hydromorphone,
Morphine,
Opiates,
Opiates,
Oxycodone,
Oxymorphone,
Urine
Extraction of Methylmalonic Acid from Serum Using ISOLUTE® SLE+ Prior to LC-MS/MS Analysis
This application note describes the extraction of methylmalonic acid from serum, prior to LC-MS/MS analysis. An effective and efficient ISOLUTE® SLE+ protocol optimized for the 200 μL capacity 96-well plate format is used. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs below 10%.
The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
Tags:
96-well plate,
Acidic,
Application Notes,
Biomarker,
Clinical,
English,
Extrahera,
LC-MS/MS,
MMA (Methylmalonic acid),
Methylmalonic acid (MMA),
Serum
Extraction of Methylmalonic Acid from Serum Using ISOLUTE® PLD+ Prior to LC-MS/MS Analysis
This application note describes a simple, effective ISOLUTE® PLD+ protocol for the extraction of methylmalonic acid (MMA) from serum, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts.
The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
Tags:
96-well plate,
Acidic,
Application Notes,
Biomarker,
Clinical,
English,
Extrahera,
LC-MS/MS,
MMA (Methylmalonic acid),
Methylmalonic acid (MMA),
Serum
Extraction of Methylmalonic Acid from Serum Using ISOLUTE® SAX Prior to LC-MS/MS Analysis
This application note describes a simple, effective protocol for the extraction of methylmalonic acid (MMA) from serum using ISOLUTE® SAX strong anion exchange solid phase extraction plates, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts.
Very clean extracts are possible due to the purely anion exchange retention mechanism available using this silica based sorbent. This minimizes the co-extraction of endogenous interferences through hydrophobic (or non-polar) retention.
The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
Tags:
96-well plate,
Acidic,
Application Notes,
Biomarker,
Clinical,
English,
Extrahera,
LC-MS/MS,
MMA (Methylmalonic acid),
Methylmalonic acid (MMA),
Serum
Extraction of Methylmalonic Acid from Serum Using EVOLUTE® EXPRESS AX Prior to LC-MS/MS Analysis
This application note describes a simple, effective protocol for the extraction of methylmalonic acid (MMA) from serum using EVOLUTE® EXPRESS AX solid phase extraction plates.
Samples are prepared using the EVOLUTE EXPRESS load-wash-elute procedure.
The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
Tags:
96-well plate,
Acidic,
Application Notes,
Biomarker,
Clinical,
English,
Extrahera,
LC-MS/MS,
MMA (Methylmalonic acid),
Methylmalonic acid (MMA),
Serum
Automated Fractionation of Extractable Petroleum Hydrocarbons (EPH) in Soil using ISOLUTE® EPH SPE Columns on Biotage® Extrahera™
This application note describes the operating conditions for the automated fractionation of Extractable Petroleum hydrocarbons (EPH) into aliphatic and aromatic (PAH) fractions using the ISOLUTE EPH SPE column in conjunction with the Extrahera automation system.
Tags:
Acenaphthene,
Acenapthalene,
Aliphatic hydrocarbons,
Anthracene,
Application Notes,
Aromatic hydrocarbons,
Benzo (g,h,i) perylene,
Benzo(a)anthracene,
Benzo(a)pyrene,
Benzo(b)fluoranthene,
Benzo(k)Fluoranthene,
Chrysene,
Column,
Dibenzo(ah)anthracene,
English,
Environmental,
Extrahera,
Fluoranthene,
Fluorene,
GC-MS,
Indeno(123-cd)pyrene,
Napthalene,
Phenanthrene,
Polyaromatic Hydrocarbons (PAH),
Pyrene,
Soil
Extraction of Phosphatidylethanol (PEth) Species from Whole Blood Using ISOLUTE® SLE+ Prior to HPLC-MS/MS Analysis
Phosphatidylethanol is an alcohol biomarker with a high degree of specificity; blood concentration of PEth correlates to the amount of alcohol consumed. This application note describes the extraction of 3 common species of PEth from whole blood using ISOLUTE® SLE+ supported liquid extraction prior to HPLC-MS/MS analysis.
The method is easily automated using the Biotage® Extrahera, and details of the automated procedure are included in the application note.
Tags:
96-well plate,
Alcohol metabolite,
Application Notes,
Clinical,
English,
Extrahera,
Forensic,
LC-MS/MS,
PEth,
Whole blood
Extraction of Methylmalonic Acid from Serum Using ISOLUTE® PPT+ Protein Precipitation Plates Prior to LC-MS/MS Analysis
This application note describes a simple ISOLUTE® PPT+ protocol for the extraction of methylmalonic acid (MMA) from serum, demonstrating high, reproducible analyte recoveries with low protein content in the extracts.
ISOLUTE PPT+ Protein Precipitation plates offer a fast and
extremely simple sample preparation approach. Serum proteins
are efficiently removed from the sample using an in-plate,
‘solvent first’, protein crash and filter procedure, which is
easily automated using the Biotage Extrahera system. Details of the automated procedure and data comparing manual and automated method performance are included.
Tags:
96-well plate,
Acidic,
Application Notes,
Biomarker,
Clinical,
English,
Extrahera,
MMA (Methylmalonic acid),
Methylmalonic acid (MMA),
Serum
Ultra-Sensitive Method for the Determination of 1,25 di-OH Vitamin D2 and 1,25 di-OH Vitamin D3 in Serum Using Supported Liquid Extraction Prior to LC-MS/MS
This application note describes a protocol for the extraction of 1,25 di-OH Vitamin D2 and 1,25 di-OH Vitamin D3 metabolites from serum using supported liquid extraction prior to LC-MS/MS detection.
A calibration range between 5 and 500 pg/mL is demonstrated using a starting volume of 0.25 mL of serum. Sensitivity is maximized through the use of a simple PTAD derivatization and formation of a methylamine complex.
The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
Tags:
1, 25 Dihydroxyvitamin D,
96-well plate,
Application Notes,
Clinical,
English,
Extrahera,
LC-MS/MS,
Serum,
Vitamin D
Extraction of 25-hydroxy Vitamin D from Serum Using ISOLUTE® PLD+ Prior to LC-MS/MS Analysis
ISOLUTE® PLD+ Protein and Phospholipid Removal plates offer a substantial improvement in extract cleanliness compared to traditional protein precipitation techniques for bioanalytical sample preparation.
This application note describes a simple, effective ISOLUTE® PLD+ protocol for the extraction of 25-hydroxy vitamin D from serum, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts.
Included in this application note are the detailed settings for implementing the method on the Biotage Extrahera automation system.
Tags:
25-OH Vitamin D2,
25-OH Vitamin D3,
25-hydroxy-vitamin D,
96-well plate,
Application Notes,
Clinical,
English,
Extrahera,
LC-MS/MS,
Serum,
Vitamin D,
Vitamins
» BROCHURES
Biotage® Extrahera™ SLE and SPE Automation System
Biotage® Extrahera™ is a powerful, user friendly automation system for Supported Liquid Extraction (SLE) and Solid Phase Extraction (SPE) based methods, in plate or column formats.
Tags:
Brochures
» CATALOGS
Analytical Sample Preparation 2019 Catalog
In this catalog product information is displayed with easy-to-follow application details and supporting documentation. Ordering information is found following each product listing.
Tags:
Catalogs
» CUSTOMER CASES
Less Labor with Automated Sample Preparation – Customer Case: Jonathan Tang, UEA
In this customer case, Jonathan Tang at University of East Anglia in the UK explains how Biotage sample preparation products have been used successfully in his studies on the human skeleton.
Tags:
Customer Cases
» MANUALS & USER GUIDES
Site Requirements Biotage® Extrahera™
This document will assist customers in the preparation
of their laboratory for a successful installation of
Biotage® Extrahera™.
Tags:
Manuals & User Guides
Biotage® Extrahera™ Safety Translations
Translated versions of the Safety chapter available in the Biotage® Extrahera™ Installation and Safety document (P/N 414157). Languages: Chinese, Danish, Dutch, Finnish, French, German, Greek, Italian, Japanese, Portuguese, Spanish, and Swedish.
Tags:
Chinese,
Danish,
Dutch,
Finnish,
French,
German,
Greek,
Italian,
Japanese,
Manuals & User Guides,
Portuguese,
Spanish,
Swedish
Biotage® Extrahera™ Getting Started Guide
Contents: System Overview; Power Failure; Manage Solvents, Sample Types, Plates, Racks, and Pipette Tips; Set Up a Method; Run Single or Multiple Methods; Maintenance; Manual Control; Troubleshooting; Switch Between 24, 48, and 96 Format; General Information
Tags:
English,
Manuals & User Guides
» POSTERS
Ultra-fast Trace Quantitation of PFAS in Drinking and Environmental Waters Using an Automated Sample Preparation and LC-MS/MS System
This poster describes an automated method for solid phase extraction of 62 PFAS compounds from drinking and environmental waters using Biotage Extrahera and EVOLUTE EXPRESS WAX SPE columns.
ASMS 2019
Tags:
Column,
English,
Environmental,
Extrahera,
LC-MS/MS,
PFAA,
PFAAs,
Perfluorobutanesulfonic acid (PFBuS),
Perfluorodecanoic acid (PFDA),
Perfluoroheptanoic acid (PFHpA),
Perfluorohexanesulfonic acid (PFHxS),
Perfluorohexanoic acid (PFHxA),
Perfluorononanoic acid (PFNA),
Perfluorooctanesulfonic acid (PFOS),
Perfluorooctanoic acid (PFOA),
Posters
Low Cost Entry for Automation in Laboratory Settings
This poster compares manual SPE (EVOLUTE EXPRESS CX) processing using the Biotage Pressure+ 96 with automated processing using Biotage Extrahera, using a suite of 98 drugs of abuse analytes in whole blood . Parameters such as the total time for extraction of 96 samples, and consistency of analyte recovery and RSD are explored.
SOFT 2019
Tags:
96-well plate,
Amitriptyline,
Anticonvulsant,
Antipsychotics,
Barbiturates,
Benzodiazepines,
Cannabinoid,
Clinical,
English,
Forensic,
Hallucinogen,
Ketamine,
LC-MS/MS,
Posters,
Stimulants,
Whole blood
Evaluation of Simplified Workflow for Hair Matrix Extraction Prior to LC-MS/MS Analysis
This poster describes an improved workflow for the analysis of
drugs of abuse from hair. Implementation of bead homogenization along with automated sample preparation allowed for simplified methodology. The direct solvent extraction approach avoids the need for pre-concentration while maintaining desired LOQs with either 200 or
400 μL of hair extract.
SOFT 2018
Tags:
96-well plate,
Amphetamines,
Benzodiazepines,
Column,
English,
Extrahera,
Forensic,
Hair,
LC-MS/MS,
Lysera,
Opiates,
Opioid,
Posters
Evaluation of Simplified Workflow for Hair Matrix Extraction Prior to LC-MS/MS Analysis
This poster describes an improved workflow for the analysis of drugs of abuse from hair.
TIAFT 2018, Ghent, Belgium
Tags:
2-OH-Et-Flurazepam,
6-acetylmorphine (MAM),
7-Aminoclonazepam,
7-Aminoflunitrazepam,
96-well plate,
Alprazolam,
Amphetamine,
Amphetamines,
Benzodiazepines,
Benzoylecgonine,
Bromazepam,
Buprenorphine,
Clonazepam,
Cocaine,
Codeine,
Column,
Diazepam,
Dihydrocodeine (DHC),
EDDP,
English,
Estazolam,
Extrahera,
Fentanyl,
Flunitrazepam,
Flurazepam,
Forensic,
Hair,
Hydrocodone,
Hydromorphone,
Ketamine,
LC-MS/MS,
Lorazepam,
Lysera,
Lysergic acid diethylamide (LSD),
MDA,
MDEA,
MDMA,
Methadone,
Methamphetamine,
Midazolam,
Morphine,
Nitrazepam,
Norbuprenorphine,
Nordiazepam,
Norfentanyl,
Norketamine,
Opiates,
Opioid,
Oxazepam,
Oxycodone,
Oxymorphone,
PCP,
Posters,
Temazepam,
Triazolam,
Zaleplone,
Zolpidem,
Zopiclone,
z-drugs,
α-OH Triazolam,
α-OH alprazolam
Drugs of Abuse Extraction from Whole Blood using Supported Liquid Extraction (SLE) and Extrahera Automation Prior to UPLC-MS/MS Analysis
This poster discusses the potential for a single extraction protocol
for various drugs of abuse classes in whole blood prior to UPLC-MS/MS analysis.
SOFT 2017, Boca Raton
also presented at SFTA, Marseille, France, 2018
Tags:
Amphetamines,
Benzodiazepines,
Cannabinoid,
Cathinones,
Clinical,
Cocaines,
Column,
Drugs of Abuse,
English,
Extrahera,
Forensic,
LC-MS/MS,
Opiates,
Posters,
THC metabolites,
Whole blood,
z-drugs
Drugs of Abuse with Multivariate Intermolecular Properties Analyzed by Polymeric Mixed-Mode Cation Exchange
This poster demonstrates that a large urine panel, comprised of 43 DOAs, from multiple drug classes, can be simultaneously screened by mixed-mode cation exchange SPE (using EVOLUTE EXPRESS CX 96 well plates) despite their disparate intermolecular traits, by thoughtfully selecting appropriate organic wash and elution conditions that simultaneously enable sample isolation and detection along with minimizing sample matrix effects.
The extraction method is automated using the Biotage® Extrahera™ Automated sample Preparation Platform.
MSACL 2017, Palm Springs
SOFT 2017, Boca Raton
Tags:
96-well plate,
Benzodiazepines,
Carbamates,
Carisoprodol,
Clinical,
Drugs of Abuse,
English,
Extrahera,
Forensic,
Gabapentin,
Hydrolysed urine,
LC-MS/MS,
Meprobamate,
Opiates,
Pain Management,
Posters,
Pregabalin,
Stimulants,
Urine
Evaluation of three beta-glucuronidase enzymes to determine the best hydrolysis conditions for urine samples in clinical toxicology and pain management
Most drugs are excreted in urine as glucuronide conjugates. Hydrolysis using a beta-glucuronidase enzyme to convert the metabolites to their “free” form for analysis increases sensitivity. Red abalone (Kura Biotech), abalone (Campbell Scientific), and recombinant (IMCSzyme) beta-glucuronidase enzymes were evaluated to determine which provided the most complete hydrolysis of glucuronide metabolites without effecting the overall recovery of non-conjugated compounds.
EVOLUTE EXPRESS CX 96-well plates were used to extract hydrolysed urine samples, and the impact of th enzymes was compared.
MSACL 2017, Palm Springs
SOFT 2017, Boca Raton
Tags:
6-acetylmorphine (MAM),
7-Aminoclonazepam,
96-well plate,
Alprazolam,
Amitriptyline,
Amphetamine,
Amphetamines,
Analgesics,
Barbiturates,
Benzodiazepines,
Benzoylecgonine,
Butalbarbital,
Carbamates,
Carisoprodol,
Chlordiazepoxide,
Clinical,
Clonazepam,
Cocaine,
Cocaines,
Codeine,
Diazepam,
Dihydrocodeine (DHC),
Drugs of Abuse,
EDDP,
English,
Fentanyl,
Forensic,
Gabapentin,
Hydrocodone,
Hydrolysed urine,
Hydromorphone,
Ketamine,
LC-MS/MS,
Lorazepam,
MDMA,
Meperidine,
Meprobamate,
Methadone,
Methamphetamine,
Morphine,
N-des-tapentadol,
Naloxone,
Norbuprenorphine,
Nordiazepam,
Norfentanyl,
Norhydrocodone,
Norketamine,
Normeperidine,
Nortryptiline,
Opiates,
Over the counter pharmaceuticals,
Oxazepam,
Oxycodone,
Oxymorphone,
Pain Management,
Pharmaceuticals,
Phencyclidine (PCP),
Phenobarbital,
Posters,
Pregabalin,
Ritalinic acid,
Secobarbital,
Stimulants,
THC metabolites,
THC-COOH,
Tapentadol,
Zolpidem,
Zolpidem-phenyl-4-COOH,
z-drugs,
α-OH alprazolam
Simultaneous Extraction of Catecholamine and Metanephrines from Urine Prior to Analysis using LC-MS/MS
This poster discusses the impact of optimization of various parts of the method development process to maximize assay performance while delivering a combined assay for the analysis of urinary catecholamines and metanephrines. The extraction method using EVOLUTE EXPRESS WCX 96-well plates is successfully automated using the Biotage® Extrahera™ Automated Sample Preparation Platform.
MSACL 2017, Palm Springs
ASMS 2017, Indianapolis
Tags:
3-methoxytyramine,
96-well plate,
Catecholamines,
Clinical,
Dopamine,
English,
Epinephrine,
Extrahera,
LC-MS/MS,
Metanephrine,
Metanephrines,
Norepinephrine,
Normetanephrine,
Posters,
Urine
Evaluation of Novel Automated Sample Preparation Compared to Manual Processing in Forensic Toxicology
This poster compares the performance of manual processing to a novel automated sample preparation system prior to GC/MS or LC-MS/MS analysis in forensic toxicology applications. Emphasis is placed on the potential for 96-well cross contamination and strategies for its elimination.
TIAFT 2016
SOFT 2016
Tags:
2-Hydroxyethylflurazepam,
2-OH-Et-Flurazepam,
6-acetylmorphine (MAM),
7-Aminoclonazepam,
7-Aminoflunitrazepam,
96-well plate,
Alprazolam,
Amphetamine,
Amphetamines,
Benzodiazepines,
Bromazepam,
Buprenorphine,
Clinical,
Clonazepam,
Codeine,
Column,
Diazepam,
Dihydrocodeine (DHC),
Drugs of Abuse,
EDDP,
English,
Estazolam,
Extrahera,
Fentanyl,
Flunitrazepam,
Flurazepam,
Forensic,
GC-MS,
Hydrocodone,
Hydromorphone,
Ketamine,
LC-MS/MS,
Lorazepam,
MDA,
MDEA,
MDMA,
Mephedrone,
Methadone,
Methamphetamine,
Midazolam,
Morphine,
Nitrazepam,
Norbuprenorphine,
Nordiazepam,
Norfentanyl,
Norketamine,
Opiates,
Opiates,
Oxazepam,
Oxycodone,
Oxymorphone,
Phencyclidine (PCP),
Posters,
Temazepam,
Triazolam,
Urine,
Whole blood,
Zaleplone,
Zolpidem,
Zopiclone,
z-drugs,
α-OH Triazolam,
α-OH alprazolam
Evaluation of Drugs of Abuse Extraction from Whole Blood Using Supported Liquid Extraction (SLE) Prior to GC/MS Analysis
This poster demonstrates a fast, reliable protocol to extract multiple drug of abuse panels from whole blood using a common supported liquid extraction methodology. This benefits laboratory workflow where multiple assays are run each day, saving both worker hours and
consumable costs.
SOFT 2015
ASMS 2016
TIAFT 2016
Tags:
2-Hydroxyethylflurazepam,
2C-B,
5-APB,
6-APB,
6-acetylmorphine (MAM),
7-Aminoclonazepam,
7-Aminoflunitrazepam,
Alprazolam,
Amobarbital,
Amphetamine,
Anhydroecgonine Methyl Ester AEME,
BZP,
Benzoylecgonine,
Bromazepam,
Butabarbital,
Butalbarbital,
Butylone,
Clinical,
Clonazepam,
Cocaethylene,
Cocaine,
Cocaines,
Codeine,
Column,
Diazepam,
Dihydrocodeine (DHC),
Drugs of Abuse,
Ecgonine Methyl Ester EME,
English,
Estazolam,
Ethylone,
Flunitrazepam,
Flurazepam,
Forensic,
GC-MS,
Hexobarbital,
Hydrocodone,
Hydromorphone,
Lorazepam,
MDA,
MDEA,
MDMA,
MDPV,
Mephedrone,
Methamphetamine,
Methcathinone,
Methedrone,
Methylone,
Midazolam,
Morphine,
Naphyrone,
Nitrazepam,
Nordiazepam,
Oxazepam,
Oxycodone,
Oxymorphone,
Pentobarbital,
Phenobarbital,
Posters,
Secobarbital,
TFMPP,
Temazepam,
Triazolam,
Whole blood,
mCPP,
pMMA,
α-OH Triazolam,
α-OH alprazolam
» PRODUCT NOTES
Comparison of Biotage® Extrahera™ vs. Manual Sample Processing Using a Vacuum Manifold: Extraction of 25-OH Vitamin D from Plasma Using ISOLUTE® SLE+
Automated sample preparation using the Biotage®Extrahera™ was compared to an equivalent manual method utilizing a vacuum manifold. Analytes were extracted from pooled stripped plasma using a supported liquid extraction procedure.
Tags:
25-OH Vitamin D2,
25-OH Vitamin D3,
25-hydroxy-vitamin D,
96-well plate,
Clinical,
English,
Extrahera,
LC-MS/MS,
Plasma,
Product Notes,
Vitamin D
Comparison of Biotage® Extrahera™ vs Manual Sample Processing Using a Vacuum Manifold: Extraction of Beta Blockers from Plasma Using ISOLUTE® SLE+
This product sheet compares automated sample preparation using the Biotage®Extrahera™ to an equivalent manual method utilizing a vacuum manifold. A selection of beta blocker drugs were extracted from pooled
stripped plasma using a supported liquid extraction procedure.
Tags:
96-well plate,
Alprenolol,
Beta blockers,
Clinical,
English,
Extrahera,
Forensic,
LC-MS/MS,
Metoprolol,
Pharma,
Pindolol,
Plasma,
Product Notes,
Propranolol,
β-blockers
Comparison of Biotage® Extrahera™ vs. Manual Sample Processing Using a Vacuum Manifold: Extraction of NSAIDs from Plasma Using ISOLUTE® SLE+
This document compares automated sample preparation using the Biotage®
Extrahera™ to an equivalent manual method utilizing a vacuum manifold. A selection of non-steroidal anti-inflammatory drugs (NSAIDS) were extracted from pooled stripped plasma using a supported liquid extraction procedure.
Tags:
96-well plate,
Clinical,
English,
Extrahera,
Forensic,
Indomethacin,
Ketoprofen,
LC-MS/MS,
NSAIDs,
Pharma,
Plasma,
Product Notes,
Sulindac
Biotage® Extrahera™ Precision and Accuracy Performance (24 position)
This document details an example of the pipetting precision and accuracy data for four specified volumes across 24 (4 x 6) positions.
Tags:
Clinical,
Column,
English,
Extrahera,
Forensic,
Gravimetric,
Pharma,
Product Notes
Biotage® Extrahera™ Precision and Accuracy Performance (96 well plate)
This document details an example of the pipetting precision and accuracy data for four specified volumes across a full 96 well plate.
Tags:
96-well plate,
Clinical,
English,
Extrahera,
Forensic,
Gravimetric,
Pharma,
Product Notes
» RELEASE NOTES
Biotage® Extrahera™ Release Notes
What’s New in Biotage® Extrahera™ 1.5.5
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English,
Release Notes
Biotage® Extrahera™ Release Notes
What’s New in Biotage® Extrahera™ 1.5.4
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Release Notes
Biotage® Extrahera™ Release Notes
What’s New in Biotage® Extrahera™ 1.5.3
Tags:
English,
Release Notes
Biotage® Extrahera™ Release Notes
What’s New in Biotage® Extrahera™ 1.5.2
Tags:
English,
Release Notes
Biotage® Extrahera™ Release Notes
What’s New in Biotage® Extrahera™ 1.5.1
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English,
Release Notes
Search all documents »
PRODUCT VIDEOS
Biotage® Extrahera™ - The Full Sequence
Biotage® Extrahera™ Presentation
Extracting Oxylipins at Karolinska Institute - a Researcher’s Perspective
WEBINARS
Biotage® Extrahera™ - Automated Sample Preparation Platform
Webinar Biotage Extrahera – Automatisches Probenverarbeitungssystem
News
27 September 2019Two New Methods for Extraction of a Steroid Hormone Panel from Human Urine
Two new application notes from Biotage describe both manual and automated procedures for sample pre-treatment and extraction of a panel of 19 steroid hormones from human urine, using EVOL...
09 July 2019"I Was Surprised that Everything Worked So Well". A Researcher on Moving to Automated Sample Prep
Antonio Checa is Assistant Professor in Prof. Craig Wheelock's lab at Biomedicum, Karolinska Institute. When moving to automated sample preparation of oxylipin samples, he decided to go with Biotag...
27 June 2019Extract THC and its Metabolites from Human Hair - New Application Note
This application note describes a procedure for sample pre-treatment and extraction of THC and metabolites from human hair, using Biotage® Lysera for matrix pulverization of the sampl...
26 June 2019A Fully-automated Method for Extraction of Opiates from Urine
This application note describes the fully-automated extraction of opiates from acid-hydrolyzed urine and non-hydrolyzed urine prior to GC/MS analysis. The methods were automated using &nb...
20 June 2019Extraction of 49 Drugs of Abuse from Human Nails - New Application Note
The testing of alternative matrices in forensic and/or clinical toxicology is gaining popularity, partly due to less invasive means of collection. Matrices such as hair or nail can ...
14 February 2019Drugs of Abuse Urine Testing White Paper Updated to Include a Further 50 Analytes
A White Paper from Biotage Abstract Analysis of drug panels in urine samples can be challenging, and the trend towards larger panels including multiple drug classes compounds the issues f...
28 August 2018Two New Application Notes for Extraction of 1-nor-9-Carboxy-Δ9-THC from Hydrolyzed Urine
These two new application notes from Biotage describe the fully automated extraction of carboxy-THC from urine, following base hydrolysis prior to GC/MS analysis. The methods were automated using B...
11 January 2018The 2018 Analytical Catalog is Here
The Biotage 2018 catalog of analytical sample preparation products for chemistry professionals is now available. This edition includes a comprehensive range of sample preparation consumables for bi...
17 July 2017Biotage Goes Direct in Italy
On July 1st, Biotage expanded its direct sales organization and opened the door to analytical customers in Italy. Account Manager Gianluca Pessoni is added to the Italian team, filling the ana...
02 May 2017ISOLUTE® SLE+ Plate Proves Itself in New Vitamin D Study
A newly published method for measuring vitamin D metabolites in human serum gives heavy credit to Biotage® Extrahera™ automated sample processor and ISOLUTE® SLE+ supported liquid ext...
20 April 2017Extraction of Vitamin B7 (Biotin) from Serum Using EVOLUTE® EXPRESS ABN
Vitamin B7 (Biotin) is a water soluble vitamin necessary for cell growth. The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum whi...
21 March 2017Extraction of Phosphatidylethanol Species from Whole Blood Using ISOLUTE® SLE+
This simple sample preparation procedure produces clean extracts, good recoveries with low RSD, and LOQ from 20 ng/mL. This method can be automated using Biotage® Extrahera™. The applicat...
20 March 2017Automated Fractionation of Extractable Petroleum Hydrocarbons (EPH) in Soil
This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE® EPH column in conjunction with the Biotage&re...
22 February 2017New Sample Preparation Findings Presented at MSACL
Four posters were presented at MSACL in Palm Springs, CA, 22-26 January 2017. P154: Method Optimization for the Low Level Detection of Vitamin B7 from Human Serum Using UPLC-MS/MS An...
17 February 2017SPE Protocol for Extraction of Urinary Catecholamines and Metanephrines
This application note describes a mixed-mode weak cation exchange SPE protocol for the extraction of catecholamines and metanephrine metabolites from urine prior to LC-MS/MS detection. The...
03 November 2016Webinar: Extrahera - Automated Sample Preparation
Thursday 3rd November 2016 11.00am Central European Time (10.00am Greenwich Mean Time) 1.00pm US Eastern Time (10.00am US Pacific Time) Register here http://view6.workcast.net/register...
27 September 2016Automated vs. Manual Sample Preparation in Forensic Toxicology
Recovery profiles from manual and automated supported liquid extraction protocols were put side by side in a study on drugs of abuse in blood and urine matrices. Data reliability in forens...
03 May 2016Six MMA Extraction Methods for Biotage® Extrahera™
The Biotage R&D Laboratory has developed six methods optimized for extracting methylmalonic acid from serum on the automated sample processing platform Biotage® Extrahera™. ...
20 April 2016Validated Method for Analysis of Urinary Catecholamines
An ISO accredited method for analysis of urinary free catecholamines by mass spectrometry was presented in a poster at MSACL 2016 in Palm Springs, USA. The method, now ...
16 March 2016Four Analytical Posters Presented at MSACL 2016
Biotage presented four new analytical posters on extraction of drugs and endogenous analytes from urine at the MSACL 2016 conference in Palm Springs, USA. The Association for Ma...
14 March 2016Ultra-Sensitive Method for Determining Vitamin D2 and D3 Metabolites in Serum
A new application from Biotage Labs describes a protocol for the extraction of 1,25 di-OH Vitamin D2 and 1,25 di-OH Vitamin D3 metabolites from serum using supported liquid extraction prior to LC-M...
26 January 2016Less Labor with Automated Sample Preparation – Customer Case
“SLE+ provided the perfect solution for removal of phospholipids, it is far less labor intensive than solid phase extraction methods and can be easily automated on the Extrahera. It has great...
05 January 20162016 Analytical Sample Preparation Catalog Released
Now available, the Biotage 2016 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...
13 October 2015Automated Extraction of Vitamin D from Serum
A new poster from Biotage outlines extraction of 25-hydroxy vitamin D from serum using ISOLUTE® PLD+ and SLE+ on a Biotage® Extrahera™ automated sample preparation platform. &nb...
15 June 2015Novel Extraction Protocol for Vitamin D
A poster from Biotage labs presented at ASMS 2015 demonstrates the applicability of ISOLUTE® PLD+ for the extraction of 25-hydroxy vitamin D from 100 or 150 μL of serum. This poster...
30 April 2015Biotage® Extrahera™ Software Tutorial
What's probably most appreciated about Biotage® Extrahera™ sample preparation automation system is the clean user interface. This video provides a software walkthrough, highlighting all t...
23 April 2015New Video - Extrahera Sample Processor
"Extrahera has been designed with just one thing in mind - to extract". See Product Manager Paul Roberts present Biotage® Extrahera™ automated system for sample preparation. ...
30 March 2015Evaluation of Extraction Methods Prior to MS Presented at MSACL 2015
Three case studies were presented by Biotage at MSACL 2015 in San Diego, CA. The studies refer to various analyte extraction methods prior to GC/MS and LC/MS.
08 January 20152015 Analytical Sample Preparation Catalog Released
Now available, the Biotage 2015 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...
12 December 2014Biotage® Extrahera™ Sample Processor Outperforms Manual Methods
A 19% improvement in relative standard deviation was recorded for a sample preparation method performed using Biotage® Extrahera™ compared to the equivalent manual method. Two st...
01 October 2014New Automated Sample Preparation System
Uppsala, Sweden, 1st October 2014 – Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, announces the launch of the new...
26 March 2011Biotage Releases Comprehensive New Catalog of Sample Preparation Products and Applications
Biotage announced the release of its new Analytical Sample Preparation Catalog. The expanded, 200-page catalog includes a range of products for Bioanalytical, Forensic, Clinical, Environmental, Agr...
10 January 2011Biotage Announces New Methods for the Extraction of Key Compounds for Clinical, Environmental and Forensic Applications
Charlotte, North Carolina USA, January 7, 2011 Biotage® (STO: BIOT), a leading supplier of tools and technology for analytical and medicinal chemistry, introduced a new series of application no...
Q&A
Extrahera and Sample Vials
01 October 2014Question:
Can I use my present sample vials on the system when working with Extrahera?
Answer:
Yes, as long as they are of the following dimensions 16 x 100 mm, 13 x 100 mm or 12 x 75 mm.
Browse more questions and answers >Solvent and Sample Tips on Extrahera
01 October 2014Question:
Can I use other automation tips on Extrahera?
Answer:
Yes, but precision and accuracy has been determined with the Biotage tips and these specifications are affected by the quality of liquid handling tips.
Browse more questions and answers >Extrahera Compatible Solvents
01 October 2014Question:
Can I add/work with my own solvents and consumables on Extrahera?
Answer:
Yes you can add your own solvents and consumables onto the system. The system is provided with most common laboratory solvents already preconfigured and has been optimized to work with Biotage consumables.
Browse more questions and answers >Applications on Extrahera
01 October 2014Question:
Will all Biotage applications transfer onto Extrahera?
Answer:
The majority of applications will transfer onto Extrahera. Some pre-treatment steps may not be automatable, and there may be limited instances where volumes are not compatible. It is advisable to gain a copy of a customer’s application/methods and discuss the requirement with a Biotage representative.
Browse more questions and answers >Printing from Extrahera
01 October 2014Question:
Can I print directly from the software on the system?
Answer:
No, pdf reports are exportable via USB, which can be stored electronically or printed from your local computer.
Browse more questions and answers >Process Individual Wells with Extrahera
01 October 2014Question:
Can I pick and choose which wells of a plate I want to process?
Answer:
Yes, in so much that you can choose to process a specific row of wells on a plate.
Browse more questions and answers >Extrahera as a Liquid Handler
01 October 2014Question:
I like the compact secure format of Extrahera. Can I use it for my general liquid handling steps too?
Answer:
Extrahera has been designed mainly to process SLE and SPE but depending on what you want to achieve, you can program a method to only perform some steps, which then essentially turns the Extrahera into a liquid handler.
Browse more questions and answers >Avoiding Cross-contamination
01 October 2014Question:
We need to avoid cross contamination at all times. How does Extrahera take care of that?
Answer:
Biotage has taken great care during the design of Extrahera to minimize the chances of cross contamination. The design incorporates a special flow-through waste plate under the extraction plate which prevents droplet splashing and contamination between samples from the Luer outlets of a plate or column. The design and robotic arm movement is programmed to minimize the chances of cross contamination at all times.
Browse more questions and answers >Extrahera Start-up Kit
01 October 2014Question:
What is included in the Extrahera Start-up kit?
Answer:
- Lifting Handles x 4
- Tip Holder x 2
- Solvent Reservoir Racks x 1
- Solvent Reservoirs x 5
- Pipette Waste Bin x 1
- Waste Kit incl. 5L waste reservoir and tubing
- Biotage Disposable Tips 1000 µL (Pk/96) x 2
- Biotage® Extrahera™ Getting Started Guide
- Biotage® Extrahera™ Installation and Safety
- Solvent inlet S1 Tubing
- Solvent inlet S2 Tubing
- Solvent inlet S3 Tubing
- Solvent inlet S4 Tubing
- Solvent inlet S5 Tubing
- Mains Cable for all regions
- Tools
- Air Regulator
- Air Hose
- Press fit Union
- Air Duct Adapter and seal
INSTRUMENTS
| Part No. | Description | Product type | Pack Size | Price | ||
|
414001 | Biotage® Extrahera | Instruments | 1 | Log in for price | |
SERVICE & SUPPORT
| Part No. | Description | Product type | Pack Size | Price | ||
|
SER-EX-FYMP | First Year Maintenance Package - Biotage® Extrahera | Service & Support | 1 | Log in for price | |
|
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SER-EX-FYMP-Q | FYMP and Qualification Package (IQOQ) - Biotage® Extrahera | Service & Support | 1 | Log in for price | |
|
|
SER-EX-IN | Installation and Familiarization - Biotage® Extrahera | Service & Support | 1 | Log in for price | |
|
|
SER-EX-IQOQ | Qualification Package (IQ/OQ) - Biotage® Extrahera | Service & Support | 1 | Log in for price | |
|
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SER-EX-SAL | Service Agreement - Limited - Biotage® Extrahera | Service & Support | 1 | Log in for price | |
|
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SER-EX-SAP | Service Agreement - Priority - Biotage® Extrahera | Service & Support | 1 | Log in for price | |
CONSUMABLES
| Part No. | Description | Product type | Pack Size | Price | ||
|
121-5202 | Collection Plate, 1 mL Square | Consumables | 50 | Log in for price | |
|
121-5203 | Collection Plate, 2 mL Square | Consumables | 50 | Log in for price | |
|
121-5208 | Collection Plate, 10 mL 24-well | Consumables | 25 | Log in for price | |
|
121-5210 | Collection Plate, 5 mL, 48-well | Consumables | 20 | Log in for price | |
|
121-5213 | Collection Plate, 2 mL Round | Consumables | 50 | Log in for price | |
|
413282 | Test Tubes (16 x 75 mm, Uncap) | Consumables | 1000 | Log in for price | |
|
414141 | 1000 µL Clear Tips, 960 tips/box, Biotage® Extrahera | Consumables | 1 | Log in for price | |
|
414574 | Test Tubes (18 x 75 mm, Uncap) | Consumables | 304 | Log in for price | |
| 416444 | 1000 µL Wide Bore, Clear Tips, 960 tips/box, Biotage® Extrahera | Consumables | 1 | Log in for price | ||
|
C40707 | Test Tubes (13 x 100 mm, Uncap) | Consumables | 1000 | Log in for price | |
|
C40708 | Test Tubes (16 x 100 mm, Uncap) | Consumables | 1000 | Log in for price | |
|
C44651 | Test Tubes (12 x 75 mm, Uncap) | Consumables | 1000 | Log in for price | |
