This application note describes a simple, effective protocol for the extraction of methylmalonic acid (MMA) from serum using EVOLUTE® EXPRESS AX solid phase extraction plates.
Samples are prepared using the EVOLUTE EXPRESS load-wash-elute procedure.
The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
Tags: 96-well plate, Acidic, Application Notes, Biomarker, Clinical, English, Extrahera, LC-MS/MS, MMA (Methylmalonic acid), Methylmalonic acid (MMA), Serum
The method described in this application note demonstrates selective extraction of the thyroid hormones T3, rT3 and T4 from serum. 200 μL of serum was extracted using the EVOLUTE® EXPRESS AX 30 mg fixed well plate format. High reproducible recoveries and low phospholipid content were observed, demonstrating limits of quantitation < 50 pg/mL.
Tags: 96-well plate, Application Notes, Clinical, English, Extrahera, LC-MS/MS, Reverse Tri-iodothyronine (rT3), Serum, T3, T4, Thyroid Hormone, Thyroxine (T4), Tri-iodothyronine (T3)
Ethyl glucuronide (EtG) and ethyl sulphate (EtS) are specific metabolites formed within the body after the ingestion of alcohol. In forensic toxicology, when tested together they provide more specific evidence of alcohol intake. This application note demonstrates the simultaneous extraction of both EtG and EtS in the same assay and represents further optimization of AN718 which showed single EtG extraction without EtS. Recoveries for EtG range from 90-105% and 80-103% for EtS across two formats of EVOLUTE AX columns with RSDs <10%. The LOQ is 10 ng/mL for EtG and 2 ng/mL for EtS across both EVOLUTE AX formats.
EVOLUTE AX, EVOLUTE, EtS, EtG, Ethyl Glucuronide, Ethyl Sulfate, SPE, Anion Excange, Urine, OASIS, Waters, Polymeric, Polmers,
Tags: Alcohol metabolite, Amphetamines, Application Notes, Column, Ethyl Sulfate (EtS), Ethyl glucuronide (EtG), Forensic, LC-MS/MS, MDA, Urine
Ethyl glucuronide (EtG, Figure 1.) is an ethanol metabolite formed by the glucuronidation of the parent molecule. The presence of EtG in urine and plasma is a useful and sensitive biomarker of alcohol intake in forensic toxicology. EtG can be detected at very low levels and is useful in distinguishing between ingested ethanol and that which is adsorbed through the skin from ethanol. This application note has been optimized on the RapidTrace+; an updated version of the current RapidTrace software which allows the system to process smaller bed heights than were previously possible. The application note shows analyte recoveries all above 97% with RSDs >10%.
EVOLUTE, Resin, EtG, Alcohol, Alcohol Biomarker, metabolite, anion exchange, Strata X, Oasis, Waters,
Tags: Alcohol metabolite, Application Notes, Column, Ethyl glucuronide (EtG), LC-MS/MS, RapidTrace, Urine
In this catalog product information is displayed with easy-to-follow application details and supporting documentation. Ordering information is found following each product listing.
EVOLUTE® EXPRESS Solid Phase Extraction products
from Biotage are designed for all your sample
preparation challenges. Whether you are working in the field of bio-analysis during drug development, clinical or forensic
toxicology, or with the diversity of samples in food
safety and environmental applications, the tools and
methods used for sample preparation have a clear
impact on productivity. This user guide contains information about choosing the correct EVOLUTE EXPRESS product for your application and optimizing your methodology for best results.
Tags: 96-well plate, Clinical, Column, English, Environmental, Food, Forensic, Manuals & User Guides, On-line SPE, Pharma
Catecholamine metabolites vanillylmandelic acid (VMA), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5 HIAA) are biomarkers for neuroblastoma and catecholamine-secreting tumors.
This poster presents optimization of the method development process to maximize analyte sensitivity in the extraction and quantitation of these metabolites from plasma. Sample preparation protocols using solid phase extraction (EVOLUTE EXPRESS ABN and AX) and supported liquid extraction (ISOLUTE SLE+) are presented.
MSACL NA 2018 Palm Springs, CA
Tags: 5-hydroxyindoleacetic acid (5-HIAA) , 96-well plate, Acidic catecholamine metabolites, Catecholamines, Clinical, English, Forensic, Homovanillic acid (HVA), LC-MS/MS, Plasma, Posters, Vanillylmandelic acid (VMA)
This poster evaluates 3 different sample preparation approaches (ISOLUTE SLE+, EVOLUTE EXPRESS ABN, EVOLUTE EXPRESS CX) for extraction of large multi-drug urine panels.
Each approach is assessed in terms of suitability for extraction of analytes with different different properties (pka, LogP etc).
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), 6-acetylmorphine (MAM), 7-Aminoclonazepam, 96-well plate, Alprazolam, Amitriptyline, Amphetamine, Amphetamines, Analgesics, Barbiturates, Benzodiazepines, Benzoylecgonine, Buprenorphine, Butalbital, Cannabinoid, Carbamate hypnotic, Carbamate muscle relaxant, Carisoprodol, Chlordiazepoxide, Clinical, Clonazepam, Cocaine, Cocaines, Codeine, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Fentanyl, Forensic, Gabapentin, Hydrocodone, Hydrolysed urine, Hydromorphone, Ketamine, LC-MS/MS, Lorazepam, MDMA, Meperidine, Meprobamate, Methadone, Methamphetamine, Morphine, N-des-tapentadol, Naloxone, Norbuprenorphine, Nordiazepam, Norfentanyl, Norhydrocodone, Norketamine, Normeperidine, Nortryptiline, Opiates, Opioid, Oxazepam, Oxycodone, Oxymorphone, Pain Management, Phencyclidine, Phenobarbital, Posters, Pregabalin, Ritalinic acid, Secobarbital, Tapentadol, Temazepam, Tramadol, Urine, Zolpidem, Zolpidem-phenyl-4-COOH, z-drugs, α-OH alprazolam
This poster investigates the selectivity of polymer-based solid phase extraction sorbents and rapid extraction of clinical matrices, matrix interference reduction and improved analyte detection.
Tags: 96-well plate, Clinical, English, Oxytocin, Peptides, Pharma, Posters, Serum, Vasopressin
This poster includes a simple Load-Wash-Elute solid phase extraction method using EVOLUTE EXPRESS WCX, for extraction of aminoglycoside antibiotics from a range of meat, milk and egg samples.
The sample preparation method is automated using Biotage Extrahera, with fast, sensitive analysis utilizing HILIC-MS/MS with MRM Spectrum Mode for ID confirmation (Shimadzu LCMS-8060).
Tags: 96-well plate, Amikacin, Aminoglycoside antibiotics, Apramycin, Dihydrostreptomycin, Eggs, English, Extrahera, Food, Gentamicin, Gentamicin C1, Gentamicin C1a, Gentamicin C2, Hygromycin B, Kanamycin, LC-MS/MS, Meat, Milk, Neomycin, Netilmicin, Paromomycin, Posters, Sisomycin, Spectinomycin, Streptomycin, Tobramycin, Veterinary drugs
This poster summarizes various sample preparation strategies for the
extraction of MMA from serum without the necessity for derivatization, prior to LC-MS/MS analysis. A range of sample preparation techniques of varying complexity were evaluated: protein precipitation, phospholipid depletion, supported liquid extraction and solid phase extraction using both silica and polymer-based mixed-mode anion exchange chemistries.
Method performance was evaluated for evaporative effects, assay recovery, ion suppression and phospholipid removal.
Tags: 96-well plate, Acidic, Clinical, English, LC-MS/MS, MMA (Methylmalonic acid), Methylmalonic acid (MMA), Posters, Serum
EVOLUTE® EXPRESS SPE columns and plates combine sorbent wettability with optimized SPE components, allowing better flow consistency and in many cases eliminating the need for SPE column conditioning; thus simplifying and reducing extraction processing. This poster compares the performance of various EVOLUTE EXPRESS column chemistries using 10-500 mg bed weights, in order to investigate whether sorbent conditioning and equilibration steps are truly required.
Tags: Acidic, Amitriptyline, Atenolol, Benzenesulfonic acid, Bretylium, Clinical, Column, Diethyldithiophosphate (DEDTP), Dimethylthiophosphate (DMTP), English, Extrahera, Forensic, Hexanesulfonic acid, Ketoprofen, LC-MS/MS, Metoprolol, Mianserin, Organophosphate Pesticide, Over the counter pharmaceuticals, Pain Management, Pharma, Pharmaceuticals, Posters, Prednisolone, Procainamide, Quaternary Amines, Quinidine, Salbutamol, Sulindac, Tricyclic Antidepressant, Urine, β-blockers
This poster compares the extraction of the ethanol metabolites (alcohol biomarkers) from urine using strong anion exchange (EVOLUTE AX) and weak anion exchange (ISOLUTE NH2) solid phase extraction columns. A comparison with a simple dilution protocol is also presented.
Tags: Alcohol metabolite, Column, English, Ethyl Sulfate (EtS), Ethyl glucuronide (EtG), Forensic, LC-MS/MS, Posters, Urine
This poster compares the use of anion and cation exchange SPE approaches for the extraction of these important clinical biomarkers.
MSACL Europe 2014
Tags: 96-well plate, Clinical, English, LC-MS/MS, Plasma, Posters, Reverse Tri-iodothyronine (rT3), Thyroid Hormone, Thyroxine (T4), Tri-iodothyronine (T3), rT3
This poster evaluates the performance of various polymer-based
ion exchange SPE chemistries for the extraction of thyroid
hormones prior to LC-MS/MS analysis.
Tags: 96-well plate, Clinical, English, LC-MS/MS, Posters, Reverse Tri-iodothyronine (rT3), Serum, T3, T4, Thyroid Hormone, Thyroxine (T4), Tri-iodothyronine (T3), rT3
This poster describes a reduced workflow solid phase extraction method for extraction of vitamin B7 (biotin) from human serum. Using EVOLUTE EXPRESS AX 96-well plates, the traditional sorbent conditioning and equilibration steps are not required, meaning the sample can be applied directly to the dry plate. Post extraction evaporation is also eliminated.
Tags: 96-well plate, Biotin, Clinical, English, LC-MS/MS, Posters, Serum, Vitamin B7, Vitamins
This poster focuses on the different strategies that can be employed when using EVOLUTE SPE sorbents (with both single and dual retention mechanisms) to reduce or eliminate residual phospholipids in sample extracts.
Tags: 96-well plate, Clinical, English, Forensic, LC-MS/MS, Pharma, Posters
This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches.
Tags: Biological fluids, English, HPLC, LC-MS/MS, Plasma, Posters
This poster describes the use of EVOLUTE® ABN and EVOLUTE® EXPRESS ABN for the fast and efficient extraction of a diverse mixture of peptides spiked into biological matrices.
Tags: 96-well plate, ACTH (1-24), Angiotensin Fragment 1-7, Angiotensin II,, Clinical, Elafin, Endorphin, English, Glycoprotein myelin, Helicobacter pylori (2-20), LC-MS/MS, Peptides, Pharma, Plasma, Posters, Serum, Vasoactive Intestinal Fragment 1-12
This poster outlines a new method for the extraction of a mixture of peptides from plasma using resin based SPE. Initial method optimization was performed using EVOLUTE ABN before comparison to the streamlined approach without plate conditioning and equilibration using EVOLUTE EXPRESS ABN. Presented at EBF 2012.
EBF, EBF 2012, Peptide, ABN, SPE, Polymer, Polymeric,
Tags: ACTH (1-24), Alpha-Endorphin, Angiotensin Fragment 1-7, Angiotensin II,, Elafin, Helicobacter pylori (2-20), LC-MS/MS, Myelin Glycoprotein fragment (35-55) rat, Peptides, Pharma, Plasma, Posters, Vasoactive Intestinal Fragment 1-12
This poster provides mixed-mode resin-based SPE sample preparation strategies for the simultaneous extraction of EtG and EtS and was presented at EBF 2012.
EtG, EtS, Poster, EBF, EBF 2012, Barcelona, LC-MS/MS, Ethyl Glucuronide, EVOLUTE, EVOLUTE EXPRESS, Polymeric, Polymer, SPE, Solid Phase Extraction,
Tags: 96-well plate, Alcohol metabolite, Column, Ethyl Sulfate (EtS), Ethyl glucuronide (EtG), Forensic, LC-MS/MS, Posters, Urine
This poster will demonstrate SPE sample preparation strategies for the simultaneous extraction of both ethyl glucuronide and ethyl sulphate from urine, allowing cleaner extraction protocols compared to traditional dilute and shoot approaches.
EtG, EtS, Urine, Poster, SOFT, Forensic, UPLC, LC-MS/MS, EVOLUTE, AX, WAX
Tags: 96-well plate, Alcohol metabolite, Column, Ethyl Sulfate (EtS), Ethyl glucuronide (EtG), Forensic, LC-MS/MS, Posters, Urine
Plasma renin activity (PRA) determination is used in the diagnosis of primary aldosteronism, an important cause of secondary hypertension (high blood pressure). Concomitant measurement of both aldo...13 August 2018
Biotage presented five new posters at the ASMS conference in San Diego this June. We've collected them all below for download. P177: Comparison of Sample Preparation Options for the Extr...24 October 2017
This application note from Biotage describes a solid phase extraction (SPE) protocol for the extraction of the peptide desmopressin from human serum prior to LC-MS/MS analysis. Desmopressi...05 July 2017
This application note describes a mixed-mode weak cation exchange SPE protocol for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) and three metanephrine metabolit...29 May 2017
Polyfluorinated compounds (PFCs) such as PFOS (perfluorooctanesulfonic acid) and PFOA (perfluorooctanoic acid) are man-made fluorosurfactants. Due to their widespread industrial use and chemical st...19 May 2017
The method described in this application note achieves high reproducible extraction recoveries of the peptides oxytocin and vasopressin from serum using a fast, simple EVOLUTE® EXPRESS Load-Was...26 April 2017
This application note describes a polymer-based strong anion exchange mixed-mode SPE protocol for the extraction of T3, rT3 and T4 from serum prior to LC-MS/MS analysis. The method described in th...20 April 2017
Vitamin B7 (Biotin) is a water soluble vitamin necessary for cell growth. The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum whi...06 December 2016
Could there be a sorbent for versatile sample preparation that doesn't need conditioning and equilibration? Actually, Biotage has developed five such sorbents in the EVOLUTE® EXPRESS family, an...02 February 2016
Five new application notes from Biotage labs provide a suite of methods for effective extraction of methylmalonic acid from serum using modern sample preparation techniques and materials from ...15 October 2015
A new application note from Biotage describes an optimized method for extraction of the catecholamines dopamine, epinephrine and norepinephrine from human plasma. The extraction method utilizes the...27 March 2015
Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...29 July 2014
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...10 July 2014
Eight new posters by Biotage Scientists and collaborating groups have been presented at ASMS 2014, Baltimore, MD, USA. P81 A Reduced Workflow Method For The Extraction Of Vitamin B7 (bi...30 June 2014
Biotage is attending EPRW (European Pesticides and Residues Workshop) 2014 30th June - 3rd July 2014, Dublin, Ireland Read more at conference website http://eprw2014.com/20 May 2014
Four new posters by Biotage R&D and Application Scientists have been presented this week at HPLC 2014, New Orleans, LA, USA. The studies focus on clean-up of biofluids using different sa...01 April 2014
Biotage is attending the Annual Meeting of the German Society for Experimental and Clinical Pharmacology and Toxicology (DGPT) 1st - 3rd April 2014, Hannover, Germany Read more at the conference ...11 January 2014
Biotage is attending the 22nd Congress French Toxicology Society19th Meeting Society of Hair Testing Annual Meeting Belgian and Luxembourg Toxicological Society 11th-13th June 2014, Bordeuax, Fran...28 August 2012
Uppsala, Sweden, 28th August, 2012 Biotage® (STO: BIOT), a leading global supplier of solutions and technology for analytical and medicinal chemistry, introduced EVOLUTE® EXPRESS, a novel ...28 August 2012
EVOLUTE EXPRESS 96-Well SPE EVOLUTE EXPRESS® represents a leap forward in high throughput solid phase extraction. The EVOLUTE EXPRESS range of 96-well SPE plates combine powerful EVOLUTE sorbe...18 April 2011
Uppsala, Sweden – Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, today announced the further expansion of its successful family of sample preparat...16 December 2010
Biotage Announces an Easy, Cost-Effective Approach to the Analysis of Ethyl Glucuronide (EtG) in Urine for Clinical and Forensic Toxicology Laboratories Uppsala, Sweden – Biotage®...23 February 2010
Uppsala, Sweden, March 2, 2010– Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, today announced the further expansion of its successful family of p...
Is there an expiry (‘best before’) date for ISOLUTE and EVOLUTE sample preparation products?
There is no fixed expiry date for these products.
All ISOLUTE and EVOLUTE products are packaged in inner metal laminate barrier bags, which provide an effective barrier to oxygen and moisture penetration. Recommended storage conditions are standard (see below).
We have no indications that the performance of non-bonded phase SPE sorbents is affected by long term storage, under recommended conditions.
We have no indications that the performance of bonded phase SPE sorbents is affected by long term storage, under recommended conditions.Browse more questions and answers >
|Part No.||Description||Product type||Pack Size||Price|
|121-9620||Tabless 1 mL Column Holder, VacMaster 96||Consumables||1||Log in for price|
|603-0001-AXG||EVOLUTE® EXPRESS AX 10 mg/1 mL (Tabless)||Consumables||100||Log in for price|
|603-0003-AXG||EVOLUTE® EXPRESS AX 30 mg/1 mL (Tabless)||Consumables||100||Log in for price|
||603-0010-PX01||EVOLUTE® EXPRESS AX 10 mg Fixed Well Plate||Consumables||1||Log in for price|
||603-0030-PX01||EVOLUTE® EXPRESS AX 30 mg Fixed Well Plate||Consumables||1||Log in for price|
|613-0006-BXG||EVOLUTE® EXPRESS AX 60 mg/3 mL (Tabless)||Consumables||50||Log in for price|
|613-0010-BXG||EVOLUTE® EXPRESS AX 100 mg/3 mL (Tabless)||Consumables||50||Log in for price|
|613-0015-CXG||EVOLUTE® EXPRESS AX 150 mg/6 mL (Tabless)||Consumables||30||Log in for price|
|613-0050-CXG||EVOLUTE® EXPRESS AX 500 mg/6 mL (Tabless)||Consumables||30||Log in for price|
|Part No.||Description||Product type||Pack Size||Price|
|PPM-A96-CH||1 mL (Tabless) Column Holder, Biotage® PRESSURE+ 96||Accessories||1||Log in for price|