Flash purification involves a simple liquid chromatography technique » Method development uses TLC as a way of deciding the parameters for the separation » Isocratic separations are easiest to develop, but gradient separations are more powerful » Software in the Isolera helps with conversion of an isocratic separation to a gradient » It is possible with the Spektra software to run step gradients » Loading options are dependent on the column type » SNAP offers the most flexibility » Care must be taken to choose the best loading option to get good purifications
Tags: Manuals & User Guides

One of the most common flash purification challenges is dealing with hard-to-dissolve crude samples. Because polar solvents cause poor chromatographic results when used as injection solvents in normal-phase flash chromatography, other sample load options are needed.
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