Oral fluid is of increasing importance as a biological matrix in drugs of abuse and related analyte testing because of the ease of sample collection, and difficulty in adulteration of samples, particularly when compared with urine.
Tags: Application Notes, Drugs of Abuse, English, Forensic, LC-MS/MS, Oral fluid , Saliva, White Papers

This application note describes a procedure for sample pre-treatment and extraction of a panel of 49 drugs of abuse from human hair, using Biotage® Lysera for matrix micropulverization, prior to clean up using ISOLUTE® SLE supported liquid extraction. The application note contains procedures optimized for 400 μL capacity column format and 200 μL and 400 μL supported liquid extraction plate formats, for higher throughput applications. The methodology delivers clean extracts and analyte recoveries mostly greater than 80% with RSDs lower than 10% for all analytes and LLOQ from 1 ng/mL.
Tags: 6-MAM, 7-Aminoclonazepam, 7-Aminoflunitrazepam, 96-well plate, Alprazolam, Amphetamine, Application Notes, Bromazepam, Buprenorphine, Clinical, Clonazepam, Cocaine, Codeine, Column, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Estazolam, Fentanyl, Flunitrazepam, Flurazepam, Hair, Hydrocodone, Hydromorphone, Ketamine, Lorazepam, Lysergic acid diethylamide (LSD), MDA, MDMA, Mephedrone, Methadone, Midazolam, Morphine, Nitrazepam, Norbuprenorphine, Nordiazepam, Norfentanyl, Norketamine, Oxazepam, Oxycodone, Oxymorphone, Phencyclidine (PCP), Temazepam, Triazolam, UPLC-MS/MS, Zaleplone, Zolpidem, Zopiclone, α-OH alprazolam

This application note describes a streamlined procedure for sample pre-treatment and extraction of a panel of 49 drugs of abuse from human hair, using Biotage® Lysera for matrix micropulverization, prior to direct transfer to clean up using ISOLUTE® SLE supported liquid extraction.
Tags: 96-well plate, Alprazolam, Amphetamine, Application Notes, Bromazepam, Buprenorphine, Clinical, Clonazepam, Cocaine, Codeine, Column, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Estazolam, Fentanyl, Flunitrazepam, Flurazepam, Hair, Hydrocodone, Hydromorphone, Ketamine, Lorazepam, Lysergic acid diethylamide (LSD), MDA, MDEA, MDMA, Mephedrone, Methamphetamine, Methedrone, Midazolam, Morphine, Nitrazepam, Norbuprenorphine, Nordiazepam, Norfentanyl, Norketamine, Oxazepam, Oxycodone, Oxymorphone, PCB, Temazepam, Triazolam, UPLC-MS/MS, Zaleplone, Zolpidem, Zopiclone

Biotage® PRESSURE+ manifolds deliver positive pressure, parallel processing for 96 well plates and column formats. The systems utilize a consistent, uniform flow of positive pressure to move both low and high viscosity liquids through SPE plates and columns. ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation and greatly matrix effects associated with biofluid analysis. SLE+, SLE, Supported Liquid Extraction, positive pressure manifold, Pressure+96, Pressure+48
Tags: Application Notes, Aqueous, Biological fluids, Column, Plasma, Post-Mortem fluids, Urine, Water, Whole blood

PRESSURE+ Declaration of Conformity
Tags: Certificates

Tags: English, Manuals & User Guides

Tags: English, Manuals & User Guides

This poster details current sample preparation strategies for aminoglycoside antibiotics in meat developed in a collaborative effort with Biotage and Tyson Foods, Inc. Springdale Corporate Laboratory to optimize analyte recovery (>70%), minimize analyte suppression and maintain acceptable method precision. FAST LC-MS 2014
Tags: Aminoglycoside antibiotics, Chicken meat, Column, English, Food, Gentamicin, LC-MS/MS, Neomycin, Posters, Spectinomycin, Streptomycin, Veterinary drugs

Biotage® PRESSURE+ manifolds deliver positive pressure, parallel processing for 96 well plates, 1 mL 3 mL and 6 mL column formats. The systems utilize a consistent, uniform flow of positive pressure to move both low and high viscosity liquids through SPE plates and columns. Each port of the PRESSURE+ manifold independently maintains constant pressure, increasing the overall reproducibility of analyte recoveries. This unique design allows for partially populated racks to be used without sacrificing extraction efficiency.
Tags: Product Notes

Oral fluid is of increasing importance as a biological matrix in drugs of abuse and related analyte testing because of the ease of sample collection, and difficulty in adulteration of samples, particularly when compared with urine.
Tags: Application Notes, Drugs of Abuse, English, Forensic, LC-MS/MS, Oral fluid , Saliva, White Papers