This application note describes a procedure for sample pre-treatment and extraction of a panel of 49 drugs of abuse from human hair, using Biotage® Lysera for matrix micropulverization, prior to clean up using ISOLUTE® SLE supported liquid extraction. The application note contains procedures optimized for 400 μL capacity column format and 200 μL and 400 μL supported liquid extraction plate formats, for higher throughput applications. The methodology delivers clean extracts and analyte recoveries mostly greater than 80% with RSDs lower than 10% for all analytes and LLOQ from 1 ng/mL.
Tags: 6-MAM, 7-Aminoclonazepam, 7-Aminoflunitrazepam, 96-well plate, Alprazolam, Amphetamine, Application Notes, Bromazepam, Buprenorphine, Clinical, Clonazepam, Cocaine, Codeine, Column, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Estazolam, Fentanyl, Flunitrazepam, Flurazepam, Hair, Hydrocodone, Hydromorphone, Ketamine, Lorazepam, Lysergic acid diethylamide (LSD), MDA, MDMA, Mephedrone, Methadone, Midazolam, Morphine, Nitrazepam, Norbuprenorphine, Nordiazepam, Norfentanyl, Norketamine, Oxazepam, Oxycodone, Oxymorphone, Phencyclidine (PCP), Temazepam, Triazolam, UPLC-MS/MS, Zaleplone, Zolpidem, Zopiclone, α-OH alprazolam

This application note describes a streamlined procedure for sample pre-treatment and extraction of a panel of 49 drugs of abuse from human hair, using Biotage® Lysera for matrix micropulverization, prior to direct transfer to clean up using ISOLUTE® SLE supported liquid extraction.
Tags: 96-well plate, Alprazolam, Amphetamine, Application Notes, Bromazepam, Buprenorphine, Clinical, Clonazepam, Cocaine, Codeine, Column, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Estazolam, Fentanyl, Flunitrazepam, Flurazepam, Hair, Hydrocodone, Hydromorphone, Ketamine, Lorazepam, Lysergic acid diethylamide (LSD), MDA, MDEA, MDMA, Mephedrone, Methamphetamine, Methedrone, Midazolam, Morphine, Nitrazepam, Norbuprenorphine, Nordiazepam, Norfentanyl, Norketamine, Oxazepam, Oxycodone, Oxymorphone, PCB, Temazepam, Triazolam, UPLC-MS/MS, Zaleplone, Zolpidem, Zopiclone

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Biotage® SPE Dry 96 and SPE Dry 96 Dual Sample Concentrators are suitable for evaporation of microplate samples across a broad range of formats. By delivering heated gas above and below each well, SPE Dry 96 systems dry samples quickly maintaining tight temperature control at user selected settings. Designed for high throughput sample processing, SPE Dry systems have simple front panel controls and use removable gas delivery assemblies for fast adjusting and cleaning.
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