This application note describes a procedure for sample
pre-treatment and extraction of a panel of 49 drugs of
abuse from human hair, using Biotage® Lysera for matrix
micropulverization, prior to clean up using ISOLUTE® SLE
supported liquid extraction. The application note contains
procedures optimized for 400 μL capacity column format and
200 μL and 400 μL supported liquid extraction plate formats,
for higher throughput applications. The methodology delivers
clean extracts and analyte recoveries mostly greater than
80% with RSDs lower than 10% for all analytes and LLOQ from
1 ng/mL.
Tags:
6-MAM,
7-Aminoclonazepam,
7-Aminoflunitrazepam,
96-well plate,
Alprazolam,
Amphetamine,
Application Notes,
Bromazepam,
Buprenorphine,
Clinical,
Clonazepam,
Cocaine,
Codeine,
Column,
Diazepam,
Dihydrocodeine (DHC),
Drugs of Abuse,
EDDP,
English,
Estazolam,
Fentanyl,
Flunitrazepam,
Flurazepam,
Hair,
Hydrocodone,
Hydromorphone,
Ketamine,
Lorazepam,
Lysergic acid diethylamide (LSD),
MDA,
MDMA,
Mephedrone,
Methadone,
Midazolam,
Morphine,
Nitrazepam,
Norbuprenorphine,
Nordiazepam,
Norfentanyl,
Norketamine,
Oxazepam,
Oxycodone,
Oxymorphone,
Phencyclidine (PCP),
Temazepam,
Triazolam,
UPLC-MS/MS,
Zaleplone,
Zolpidem,
Zopiclone,
α-OH alprazolam
This application note describes a streamlined procedure for
sample pre-treatment and extraction of a panel of 49 drugs
of abuse from human hair, using Biotage® Lysera for matrix
micropulverization, prior to direct transfer to clean up using
ISOLUTE® SLE supported liquid extraction.
Tags:
96-well plate,
Alprazolam,
Amphetamine,
Application Notes,
Bromazepam,
Buprenorphine,
Clinical,
Clonazepam,
Cocaine,
Codeine,
Column,
Diazepam,
Dihydrocodeine (DHC),
Drugs of Abuse,
EDDP,
English,
Estazolam,
Fentanyl,
Flunitrazepam,
Flurazepam,
Hair,
Hydrocodone,
Hydromorphone,
Ketamine,
Lorazepam,
Lysergic acid diethylamide (LSD),
MDA,
MDEA,
MDMA,
Mephedrone,
Methamphetamine,
Methedrone,
Midazolam,
Morphine,
Nitrazepam,
Norbuprenorphine,
Nordiazepam,
Norfentanyl,
Norketamine,
Oxazepam,
Oxycodone,
Oxymorphone,
PCB,
Temazepam,
Triazolam,
UPLC-MS/MS,
Zaleplone,
Zolpidem,
Zopiclone
Biotage® SPE Dry 96 and SPE Dry 96 Dual Sample Concentrators are suitable for evaporation of microplate samples across a broad range of formats. By delivering heated gas above and below each well, SPE Dry 96 systems dry samples quickly maintaining tight temperature control at user selected settings. Designed for high throughput sample processing,
SPE Dry systems have simple front panel controls and use removable gas delivery assemblies for fast adjusting and cleaning.
Tags:
English,
Product Notes
These two application notes describe alternative approaches to the extraction of a suite of regulated veterinary growth promoters (including steroids, stilbenes and resorcylic acid lactones) from a...
27 September 2019Two new application notes from Biotage describe both manual and automated procedures for sample pre-treatment and extraction of a panel of 19 steroid hormones from human urine, using EVOL...
18 September 2019The application note demonstrates an efficient sample prep workflow for horse hair samples utilizing the Biotage® Lysera for matrix pre-treatment, ISOLUTE® SLE+ for extraction and the Biota...
27 June 2019This application note describes a procedure for sample pre-treatment and extraction of THC and metabolites from human hair, using Biotage® Lysera for matrix pulverization of the sampl...
26 June 2019This application note describes the fully-automated extraction of opiates from acid-hydrolyzed urine and non-hydrolyzed urine prior to GC/MS analysis. The methods were automated using &nb...
20 June 2019The testing of alternative matrices in forensic and/or clinical toxicology is gaining popularity, partly due to less invasive means of collection. Matrices such as hair or nail can ...
24 April 2019This application note demonstrates the applicability of our newest sample preparation consumable, ISOLUTE® HYDRO DME+ for in-situ hydrolysis and clean-up of urine samples, for the analysis of a...
04 February 2019This video outlines Application AN894, where angiotensin and aldosterone are extracted in the same process using EVOLUTE® EXPRESS ABN.
06 December 2018These two new application notes from Biotage describe procedures for sample pre-treatment and extraction of a panel of 49 drugs of abuse from human hair, using Biotage® Lysera for matrix m...
14 September 2018Plasma renin activity (PRA) determination is used in the diagnosis of primary aldosteronism, an important cause of secondary hypertension (high blood pressure). Concomitant measurement of both aldo...
24 November 2017This application note describes a supported liquid extraction protocol for the extraction of three acidic catecholamine metabolites (vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacet...
19 May 2017The method described in this application note achieves high reproducible extraction recoveries of the peptides oxytocin and vasopressin from serum using a fast, simple EVOLUTE® EXPRESS Load-Was...
15 February 2017This application note describes the extraction of benzodiazepine compounds from oral fluid matrix collected with the NeoSal™ devices, prior to GC/MS analysis. The protocol described ...
10 February 2017The method described in this application from Biotage Labs demonstrates high, reproducible recovery of teicoplanin from human plasma. Samples were extracted using an EVOLUTE® EXPRESS ABN 30 mg ...
08 February 2017This application note describes the extraction of cocaine and selected metabolites from oral fluid matrix following the collection with the NeoSal™ devices, prior to GC/MS analysis. ...
12 January 2017This application note describes the extraction of opiate compounds from oral fluid matrix collected using the NeoSal™ devices, prior to GC/MS analysis. This application note des...
27 September 2016Recovery profiles from manual and automated supported liquid extraction protocols were put side by side in a study on drugs of abuse in blood and urine matrices. Data reliability in forens...
25 November 2014This application note describes the extraction of 47 drugs of abuse from oral fluid matrix after sampling via Quantisal collection devices prior to UPLC-MS/MS analysis. Oral fluid collecti...
23 October 2014A simplified method for screening metanephrine and normetanephrine proves as effective as the current gold standard. It is anticipated that this time-saving and sensitive SPE-LC-ESI-MS/MS...
Question:
Answer:
On average, an extraction can take anywhere from 15 - 20 minutes, with dirtier samples taking longer. The total run time will also be a function of the number of Pre-wet and Rinse steps that have been programmed into the method.
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SD-9600-DHS-EU | Biotage® SPE Dry 96 Sample Concentrator System 220V | Instruments | 1 | Log in for price | |
SD-9600-DHS-NA | Biotage® SPE Dry 96 Sample Concentrator System 110V | Instruments | 1 | Log in for price | ||
SD-9600-DHS-T-EU | Biotage® SPE Dry 96 System PTFE (Top Head Only) 220V | Instruments | 1 | Log in for price | ||
SD-9600-DHS-T-NA | Biotage® SPE Dry 96 System PTFE (Top Head Only) 110V | Instruments | 1 | Log in for price | ||
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SD2-9600-DHS-EU | Biotage® SPE Dry 96 Dual Sample Concentrator System 220V | Instruments | 1 | Log in for price | |
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SD2-9600-DHS-NA | Biotage® SPE Dry 96 Dual Sample Concentrator System 110V | Instruments | 1 | Log in for price | |
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SD2-9600-DHS-T-EU | Biotage® SPE Dry 96 Dual System PTFE (Top Head Only) 220V | Instruments | 1 | Log in for price | |
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SD2-9600-DHS-T-NA | Biotage® SPE Dry 96 Dual System PTFE (Top Head Only) 110V | Instruments | 1 | Log in for price |