Three protected carbohydrate derivatives, 1,2:5,6-Di-O-Isopropylidene- α-D-glucofuranose, Methyl 4,6-O-Benzylidene-α-D-glucopyranose, and 1,2-O-Isopropylidene-α-D-glucofuranose were successfully separated and detected, using a Biotage® SNAP KP-Sil cartridge on a Biotage Isolera™ system with the Biotage® Evaporative Light-Scattering Detector (ELSD) attached.
Tags: Application Notes

Three sterols, (+)-dihydrocholesterol, β-estradiol-3-benzoate, and β-estradiol were successfully separated, detected, and isolated using a Biotage® SNAP KP-Sil cartridge attached to a Biotage Isolera™ One system connected to Biotage® Evaporative Light-Scattering Detector (ELSD).
Tags: Application Notes

Introduction to the Detector, Site Requirements and Specifications, Installing the Detector, Using the Detector, Optimizing Performance, Troubleshooting and Diagnostics, Error Information, Maintenance, and Parts and Materials for Maintenance.
Tags: Manuals & User Guides

Prepare the Detector for a Run, Operational Parameters, Hints and Tips, and Disconnect and Reconnect the Detector.
Tags: Manuals & User Guides

Flash purification involves a simple liquid chromatography technique » Method development uses TLC as a way of deciding the parameters for the separation » Isocratic separations are easiest to develop, but gradient separations are more powerful » Software in the Isolera helps with conversion of an isocratic separation to a gradient » It is possible with the Spektra software to run step gradients » Loading options are dependent on the column type » SNAP offers the most flexibility » Care must be taken to choose the best loading option to get good purifications
Tags: Manuals & User Guides