Three protected carbohydrate derivatives, 1,2:5,6-Di-O-Isopropylidene-
α-D-glucofuranose, Methyl 4,6-O-Benzylidene-α-D-glucopyranose, and
1,2-O-Isopropylidene-α-D-glucofuranose were successfully separated and
detected, using a Biotage® SNAP KP-Sil cartridge on a Biotage Isolera™ system
with the Biotage® Evaporative Light-Scattering Detector (ELSD) attached.
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Application Notes
Three sterols, (+)-dihydrocholesterol, β-estradiol-3-benzoate, and
β-estradiol were successfully separated, detected, and isolated using a
Biotage® SNAP KP-Sil cartridge attached to a Biotage Isolera™ One system connected to Biotage® Evaporative Light-Scattering Detector (ELSD).
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Application Notes
Introduction to the Detector, Site Requirements and Specifications, Installing the Detector, Using the Detector, Optimizing Performance, Troubleshooting and Diagnostics, Error Information, Maintenance, and Parts and Materials for Maintenance.
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Manuals & User Guides
Prepare the Detector for a Run, Operational Parameters, Hints and Tips, and Disconnect and Reconnect the Detector.
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Manuals & User Guides
Flash purification involves a simple liquid chromatography technique
» Method development uses TLC as a way of deciding the parameters for the
separation
» Isocratic separations are easiest to develop, but gradient separations are
more powerful
» Software in the Isolera helps with conversion of an isocratic separation to
a gradient
» It is possible with the Spektra software to run step gradients
» Loading options are dependent on the column type
» SNAP offers the most flexibility
» Care must be taken to choose the best loading option to get good
purifications
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Manuals & User Guides
Biotage® SNAP Ultra flash cartridges allow you to load your samples in seven different ways. No matter what you are purifying - there is always a solution to get the best possible separati...
21 October 2014Plugging an evaporative light scattering detector (ELSD) into your flash chromatography system can greatly improve detection of non volatile compounds. This is shown in two applications on flash ch...
06 August 2014Fatty acids and their esters are found in many biological substrates. Many of these fats are unhealthy (e.g. saturated fats) while others have been found to be beneficial (e.g. unsaturated, polyuns...
29 July 2014Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
Part No. | Description | Product type | Pack Size | Price | ||
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ISO-ELSD-A120 | Biotage ELSD-A120 Detector System, Complete with tubing | Instruments | 1 | Log in for price |
Part No. | Description | Product type | Pack Size | Price | ||
SER-ELSD-FYMP | First Year Maintenance Package - Biotage® ELSD-A120 | Service & Support | 1 | Log in for price |
Part No. | Description | Product type | Pack Size | Price | ||
414126 | Biotage A120 ELSD Assembly Kit | Accessories | 1 | Log in for price |
Part No. | Description | Product type | Pack Size | Price | ||
ELSD-0890-0300 | Analogue Output Cable | Spare parts | 1 | Log in for price | ||
ELSD-0890-0315 | Waste Hose (Tygon) 7cm | Spare parts | 1 | Log in for price | ||
ELSD-0890-0320 | Solvent Waste Container (500ml) | Spare parts | 1 | Log in for price | ||
ELSD-0890-0345 | I/O Cable | Spare parts | 1 | Log in for price | ||
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ELSD-0890-0390 | ELSD Nebuliser | Spare parts | 1 | Log in for price | |
ELSD-0890-0395 | Leak Sensor Assembly | Spare parts | 1 | Log in for price |