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Biotage ZIP® Sphere
High Performance, Low Pressure Flash Cartridge
Biotage ZIP® Sphere delivers better purification and improved throughput than comparable flash cartridges. The silica surface area has been increased by 40%, meaning higher loading capacity, improved separations, and reduced purification time and solvent use.
| Sizes | 5g, 10g, 30g, 45g, 80g, 120g |
| Tube Material | Inert medical-grade polypropylene |
| Frit Material | Inert polypropylene |
| Pressure Rating | 0-100 psi / 7 bar |
| Media | Biotage® KP-Sphere - 60μ spherical silica |
| Resolution | Minimum 1500 N/m (plates per meter) |
| Compabability | Universal Luer connections |
» APPLICATION NOTES
Reversed-Phase Flash Purification
Normal-phase flash chromatography1 has been widely adopted as the method of choice for separation of product mixtures and reaction by-products. One of the most significant developments in this
area concerns the practical separation of polar molecules. Reversed-phase purification is a modification of normalphase
chromatography that provides an efficient mechanism for the separation of polar compounds.
Tags: Application Notes,
Brochures,
English,
Manuals & User Guides
» BROCHURES
Organic Chemistry - Products from Start to Finish
Biotage has developed tools for every
step of the organic process, with the entire workflow in mind.
This dedicated suite of products vastly expands the
range of options in order to truly accelerate discoveries
of new molecules for future innovations.
Tags: Brochures
Flash Purification Consumables - A Guide to Cartridges & Method Development
Flash purification is a separation technique developed in 1978 by Professor W.C. Still that uses a stationary phase (a column or cartridge filled with an insoluble solid support) and a mobile phase (elution solvent mixture) to separate and purify a mixture of organic compounds.
Tags: Brochures
Reversed-Phase Flash Purification
Normal-phase flash chromatography1 has been widely adopted as the method of choice for separation of product mixtures and reaction by-products. One of the most significant developments in this
area concerns the practical separation of polar molecules. Reversed-phase purification is a modification of normalphase
chromatography that provides an efficient mechanism for the separation of polar compounds.
Tags: Application Notes,
Brochures,
English,
Manuals & User Guides
» MANUALS & USER GUIDES
Biotage Flash Cartridge User Guide - The Definitive Cartridge User Guide
The definitive guide to flash chromatography. This document presents in detail the broad selection of ways that materials for purification can be loaded onto a flash cartridge.
Tags: Manuals & User Guides
Method Development in Flash Purification
Flash purification involves a simple liquid chromatography technique
» Method development uses TLC as a way of deciding the parameters for the
separation
» Isocratic separations are easiest to develop, but gradient separations are
more powerful
» Software in the Isolera helps with conversion of an isocratic separation to
a gradient
» It is possible with the Spektra software to run step gradients
» Loading options are dependent on the column type
» SNAP offers the most flexibility
» Care must be taken to choose the best loading option to get good
purifications
Tags: Manuals & User Guides
Reversed-Phase Flash Purification
Normal-phase flash chromatography1 has been widely adopted as the method of choice for separation of product mixtures and reaction by-products. One of the most significant developments in this
area concerns the practical separation of polar molecules. Reversed-phase purification is a modification of normalphase
chromatography that provides an efficient mechanism for the separation of polar compounds.
Tags: Application Notes,
Brochures,
English,
Manuals & User Guides
» MATERIAL SAFETY DATA SHEETS
Safety Data Sheet KP Sphere
Laboratory chemical
Not for therapeutic or diagnostic use
Tags: English,
Material Safety Data Sheets
Safety Data Sheet HP Sphere
Laboratory chemical
Not for therapeutic or diagnostic use
Tags: English,
Material Safety Data Sheets
» PRODUCT NOTES
Why Reusing Flash Cartridges is Bad Science
To save money on consumables, many chemists choose to reuse
silica flash cartridges. This is true but risks purification results because chromatographic separation performance will change from run to run which reduces purification quality, especially in normal phase systems.
Regardless of the cartridge brand used, repeated use of silica flash cartridges results in loss of compound resolution and therefore fraction purity.
Tags: Product Notes
Biotage ZIP® Sphere
Biotage ZIP Sphere delivers better purification and improved throughput than comparable flash cartridges. The silica surface area has been increased by 40%, meaning higher loading capacity, improved separations, and reduced purification time and solvent use.
Tags: Product Notes
Search all documents »
INSTRUCTION VIDEOS
Isolera™ Tutorial
WEBINARS
Reversed Phase Purification: Bridging the Gap From Flash to Prep HPLC
News
09 August 2018Webinar: A Roadmap to Successful Flash Chromatography
Want to improve your flash chromatography skills? Learn all the tricks of the trade in this one-hour webinar with flash guru Bob Bickler. For a dedicated synthetic chemist, the goal ...
31 May 2017Media Porosity, Why Should I Care?
"Normal-phase silica has, on average, 50 to 60 Å pores (and a surface area of 500 m²/g). However, for small-molecule reversed-phase chromatography to be useful, the average pore siz...
13 November 2015Scaling Up Flash Purification – Easy as 1, 2, 3, 4
This blog post on the Flash Purification Blog discusses the process of simplified flash purification scale-up. For many chemists performing bench-scale organic synthesis, flash column chro...
01 September 2015New Blog Post: How do I Choose the Right Column Size for Purification by Flash Chromatography?
Techincal specialist Bob Bickler goes through a good method to optimize column size for your purification in his latest blog post on The Flash Purification Blog. "In all my years of work...
30 June 2015Tutorial Video – How to Load Flash Chromatography Cartridges
Biotage® SNAP Ultra flash cartridges allow you to load your samples in seven different ways. No matter what you are purifying - there is always a solution to get the best possible separati...
04 December 2014Why Reusing Flash Cartridges is Bad Science
Regardless of the cartridge brand used, repeated use of silica flash cartridges results in loss of compound resolution and fraction purity. To save money on consumables, many chemists choo...
20 November 2014The Definitive Flash Cartridge Guide
Which cartridge should I use? How do different silica gels influence my purification? What is the correlation between the Rf value and column volume? What's the difference between isocratic and ste...
12 September 2014Isolera™ Used in Recent Natural Products Study
The capabilities of Biotage flash chromatography systems for providing pure compounds from natural products is confirmed in an extensive Korean study of active compounds in the medicinal herb&...
06 August 2014Difficult Fractionation of Lipids Made Easy with Isolera™ Dalton Flash Purification
Fatty acids and their esters are found in many biological substrates. Many of these fats are unhealthy (e.g. saturated fats) while others have been found to be beneficial (e.g. unsaturated, polyuns...
29 July 2014Free Shipping on EU Web Orders
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
14 March 2014The Lines Between Purification and Analysis are Blurring…
By Dr. Greg Saunders, EU Marketing Manager for Organic and Peptide Chemistry at Biotage Liquid chromatography (LC) is used for two different purposes – analytical work and purification &ndas...
18 October 2011Purifying the First Hundred Grams
Preparative FLASH chromatography expedites the production of clinical trial compounds. Tobi Williams is an application chemist specializing in pilot and production purification at Biotag...
Isolera™ Prime
Isolera™ Prime is a value based system which recommends appropriate cartridges and calculates the best solvent conditions and gradients for your purifications. It has solvent pumps, automated fraction collection as standard and delivers much more than just the basics. Solvent use is minimized and advanced compound detection makes sure that your purified compound is delivered at the end of the run. We belive in providing value, so Isolera Prime systems embrace ACI™, a revolutionary advancement, converting regular flash from simple purification to the fastest, greenest, and most economical way to reliably isolate pure compounds for organic synthesis. ACI offerred as standard on Isolera Prime.
Isolera™ Dalton
Isolera™ Dalton is the most advanced and innovative flash purification system available, designed by people who understand flash chromatography for the chemists who need it.
"Pure convenience! Just pure convenience! It lets us perform purification and analysis at the same time. It gives us tremendous power to isolate unknown compounds.”
- Hiroki Nishii, Chugai Pharmaceutical’s Kamakura Research Laboratories
Biotage ZIP® Sphere cartridges contain Biotage® KP-Sphere™, a proprietary spherical silica which is manufactured under a new process that delivers 40% more surface area (700 m2/g), no fines, and remarkably low back pressure. Typical flash cartridges use silica with a surface area of 500 m2/g and irregular particle sizes. The smallest particles or fines cause higher back pressure, lower flow rates and lower capacity.
Advantages
- High sample load compared to irregular silica particles
- Time to purify is reduced
- Less solvent required
- Reduced purification costs
Available in sizes from 5 g to 120 g
CONSUMABLES
| Part No. | Description | Product type | Pack Size | Price | ||
|
445-0500-DZ-20 | Biotage ZIP® Sphere Silica cartridges 5 g, 20/cs | Consumables | 20 | Log in for price | |
|
|
445-1000-EZ-20 | Biotage ZIP® Sphere Silica cartridges 10 g, 20/cs | Consumables | 20 | Log in for price | |
|
445-120G-UZ-20 | Biotage ZIP® Sphere Silica cartridges 120 g, 20/cs | Consumables | 20 | Log in for price | |
|
445-3000-FZ-20 | Biotage ZIP® Sphere Silica cartridges 30 g, 20/cs | Consumables | 20 | Log in for price | |
|
445-4500-SZ-20 | Biotage ZIP® Sphere Silica cartridges 45 g, 20/cs | Consumables | 20 | Log in for price | |
|
445-8000-JZ-20 | Biotage ZIP® Sphere Silica cartridges 80 g, 20/cs | Consumables | 20 | Log in for price | |