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EVOLUTE® HYDRO

Solid Phase Extraction Columns and Plates with In-Well Hydrolysis Capability

600-0030-PZ01
600-0003-AZG 601-0003-AZG 610-0006-BZG 610-0006-BZG 610-0015-CZG 610-0015-CZG 611-0015-CZG 611-0015-CZG 600-0003-AZG 601-0030-PZ01

EVOLUTE® HYDRO solid phase extraction columns and 96-well plates combine robust, high quality EVOLUTE SPE sorbents with Hydro frit technology, enabling effective in-situ in-well hydrolysis of urine samples. Sample clean-up is performed in the same column or well, without the need to transfer samples, saving time and reducing risky sample handling steps.

Streamlined Sample Processing
The columns and 96-well plates contain the Hydro frit technology system, which reliably holds up sample and hydrolysis enzyme during incubation at elevated temperatures, and reduces clogging during subsequent clean up steps.

By combining Hydro frit technology with water wettable EVOLUTE sorbents, EVOLUTE HYDRO SPE products allow the hydrolyzed sample to be loaded directly onto the SPE bed for clean-up. Conditioning and equilibration steps are eliminated, again saving time and reducing costs.

 EVOLUTE hydro schematic

Eliminating the need for off-line hydrolysis leads to significant time saving and reduction of sample handling, reducing  potential errors and sample losses. 

10 mg formats also allow analyte elution in very low volumes, allowing for no-blow down procedures.

 

Reduced Processing Time
Compared to traditional SPE procedures, EVOLUTE® HYDRO can reduce sample processing time by 20 minutes for 96 samples.

EVOLUTE hydro process time

Automated extraction on Biotage® Extrahera™ using traditional SPE takes 53 minutes for 96 samples. With EVOLUTE® HYDRO this time is reduced to 33 minutes.
[30 mg 96-well plate format, using equivalent extraction methodology. Full procedure including hydrolysis, eluent evaporation and reconstitution: 143 mins (traditional SPE) 123 mins (HYDRO SPE)].

No Compromise on Hydrolysis Efficiency

Because the HYDRO frit technology holds all of the sample above the sorbent bed during the hydrolysis process, hydrolysis efficiency when using EVOLUTE HYRO SPE columns and plates is equivalent to off-line hydrolysis under the same conditions. Existing hydrolysis procedures can easily be transferred to EVOLUTE HYDRO SPE products.

Excellent Sample Clean-up
Whether extracting large multidrug panels or performing more targeted applications, EVOLUTE HYDRO SPE products remove matrix components and residual hydrolysis enzyme, leading to cleaner extracts and reduced matrix effects in LC-MS/MS.

CONSUMABLES

Part No. Description Product type Pack Size Price
600-0001-AZ EVOLUTE® HYDRO ABN 10 mg/1 mL Consumables 100 Log in for price
600-0001-AZG EVOLUTE® HYDRO ABN 10 mg/1 mL (Tabless) Consumables 100 Log in for price
600-0003-AZ EVOLUTE® HYDRO ABN 30 mg/1 mL Consumables 100 Log in for price
600-0003-AZG 600-0003-AZG EVOLUTE® HYDRO ABN 30 mg/1 mL (Tabless) Consumables 100 Log in for price
600-0010-PZ01-old 600-0010-PZ01 EVOLUTE® HYDRO ABN 10 mg Fixed Well Plate Consumables 1 Log in for price
600-0030-PZ01 600-0030-PZ01 EVOLUTE® HYDRO ABN 30 mg Fixed Well Plate Consumables 1 Log in for price
601-0001-AZ EVOLUTE® HYDRO CX 10 mg/1 mL Consumables 100 Log in for price
601-0001-AZG EVOLUTE® HYDRO CX 10 mg/1 mL (Tabless) Consumables 100 Log in for price
601-0003-AZ EVOLUTE® HYDRO CX 30 mg/1 mL Consumables 100 Log in for price
601-0003-AZG EVOLUTE® HYDRO CX 30 mg/1 mL (Tabless) Consumables 100 Log in for price
601-0010-PZ01 EVOLUTE® HYDRO CX 10 mg Fixed Well Plate Consumables 1 Log in for price
601-0030-PZ01 601-0030-PZ01 EVOLUTE® HYDRO CX 30 mg Fixed Well Plate Consumables 1 Log in for price
610-0006-BZ EVOLUTE® HYDRO ABN 60 mg/3 mL Consumables 50 Log in for price
610-0006-BZG 610-0006-BZG EVOLUTE® HYDRO ABN 60 mg/3 mL (Tabless) Consumables 50 Log in for price
610-0015-CZ EVOLUTE® HYDRO ABN 150 mg/6 mL Consumables 30 Log in for price
610-0015-CZG 610-0015-CZG EVOLUTE® HYDRO ABN 150 mg/6 mL (Tabless) Consumables 30 Log in for price
611-0006-BZ EVOLUTE® HYDRO CX 60 mg/3 mL Consumables 50 Log in for price
611-0006-BZG EVOLUTE® HYDRO CX 60 mg/3 mL (Tabless) Consumables 50 Log in for price
611-0015-CZ EVOLUTE® HYDRO CX 150 mg/6 mL Consumables 30 Log in for price
611-0015-CZG 611-0015-CZG EVOLUTE® HYDRO CX 150 mg/6 mL (Tabless) Consumables 30 Log in for price