- Sample Preparation
- ISOLUTE® PLD+ Protein and Phospholipid Removal Products
Sample Preparation
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-
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ISOLUTE® PLD+ Protein and Phospholipid Removal Products
Effortlessly Enhance Analyte Sensitivity
ISOLUTE® PLD+ Protein and Phospholipid Removal Plates and Columns provide a very effective but extremely simple sample clean-up for LC-MS/MS analysis. Requiring next to no method development, ISOLUTE PLD+ can be integrated quickly and easily into routine workflow, increasing productivity and reducing instrument downtime.
Download the Product Note for more information.
» APPLICATION NOTES
Extraction of Methylmalonic Acid from Serum Using ISOLUTE® PLD+ Prior to LC-MS/MS Analysis
This application note describes a simple, effective ISOLUTE® PLD+ protocol for the extraction of methylmalonic acid (MMA) from serum, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts.
The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
Tags: 96-well plate,
Acidic,
Application Notes,
Biomarker,
Clinical,
English,
Extrahera,
LC-MS/MS,
MMA (Methylmalonic acid),
Methylmalonic acid (MMA),
Serum
Extraction of 25-hydroxy Vitamin D from Serum Using ISOLUTE® PLD+ Prior to LC-MS/MS Analysis
ISOLUTE® PLD+ Protein and Phospholipid Removal plates offer a substantial improvement in extract cleanliness compared to traditional protein precipitation techniques for bioanalytical sample preparation.
This application note describes a simple, effective ISOLUTE® PLD+ protocol for the extraction of 25-hydroxy vitamin D from serum, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts.
Included in this application note are the detailed settings for implementing the method on the Biotage Extrahera automation system.
Tags: 25-OH Vitamin D2,
25-OH Vitamin D3,
25-hydroxy-vitamin D,
96-well plate,
Application Notes,
Clinical,
English,
Extrahera,
LC-MS/MS,
Serum,
Vitamin D,
Vitamins
Extraction of a Range of Acidic, Basic and Neutral Drugs from Plasma Using ISOLUTE® PLD+ Plates Prior to LC-MS/MS Analysis
This application note describes the use of the ISOLUTE® PLD+ Protein and Phospholipid Removal Plate for clean-up of a range of acidic, basic and neutral drugs from plasma. ISOLUTE® PLD+ Protein and Phospholipid Removal Plates are suitable for clean-up of a range of analytes with widely differing functionality and polarity characteristics from plasma, giving high recoveries, good reproducibility and excellent extract cleanliness.
Tags: 96-well plate,
Acetaminophen,
Acidic,
Amitriptyline,
Application Notes,
Atenolol,
Basic,
Bretylium,
Brompheniramine,
Clinical,
English,
Fluoxetine,
Forensic,
Ketoprofen,
LC-MS/MS,
Metoprolol,
Mianserin,
NSAIDs,
Naltrexone,
Neutral,
Pharma,
Plasma,
Procainamide,
Quinidine,
Ranitidine,
Salbutamol,
Sulindac,
Tricyclic Antidepressant,
p-toluamide,
β-blockers
» CATALOGS
Analytical Sample Preparation 2018 Catalog
In this catalog product information is displayed with easy-to-follow application details and supporting documentation.
Ordering information is found following each product listing.
Tags: Catalogs
» POSTERS
Comparison of Sample Preparation Strategies for the Extraction of Methylmalonic Acid Prior to LC-MS/MS Analysis
This poster summarizes various sample preparation strategies for the
extraction of MMA from serum without the necessity for derivatization, prior to LC-MS/MS analysis. A range of sample preparation techniques of varying complexity were evaluated: protein precipitation, phospholipid depletion, supported liquid extraction and solid phase extraction using both silica and polymer-based mixed-mode anion exchange chemistries.
Method performance was evaluated for evaporative effects, assay recovery, ion suppression and phospholipid removal.
MSACL 2016
Tags: 96-well plate,
Acidic,
Clinical,
English,
LC-MS/MS,
MMA (Methylmalonic acid),
Methylmalonic acid (MMA),
Posters,
Serum
Evaluation of 25-hydroxy Vitamin D Extraction using Phospholipid Depletion Plate Technology and Method Comparison using Automated Sample Preparation
This poster demonstrates the use of a novel protein and phospholipid depletion plate, for the extraction of 25-hydroxy vitamin D from serum. The extraction protocol was ultimately transferred to an SPE automation platform and method performance versus manual processing was compared.
ASMS 2015
Tags: 25-OH Vitamin D2,
25-OH Vitamin D3,
25-hydroxy-vitamin D,
96-well plate,
Biomarker,
Clinical,
English,
Extrahera,
LC-MS/MS,
Posters,
Serum,
Vitamin D,
Vitamins
Extraction Comparison of 25-hydroxy Vitamin D Using Supported Liquid Extraction and Phospholipid Depletion Plate Technology
This poster describes a comparison of 25-hydroxy Vitamin D Extraction using Supported Liquid Extraction and Phospholipid Depletion Plate Technology using Manual and Automated Sample Preparation prior to LC/MS Analysis. It was presented at MSACL 2015, San Diego
Tags: 25-OH Vitamin D2,
25-OH Vitamin D3,
25-hydroxy-vitamin D,
96-well plate,
Clinical,
English,
LC-MS/MS,
Posters,
Serum,
Vitamin D,
Vitamins
Evaluation of a Novel 96-well Filter Plate for the Effective Removal of Serum Proteins and Phospholipids prior to LC-MS/MS Analysis
This poster presents the ISOLUTE PLD+ Protein and Phospholipid removal plate, highlighting its ease of use and excellent performance with respect to sample clean up, analyte recovery and elimination of back pressure build up in the UPLC system.
IMSC 2014
Tags: 96-well plate,
Acetaminophen,
Acidic,
Amitriptyline,
Atenolol,
Basic,
Bretylium,
Brompheniramine,
Clinical,
English,
Fluoxetine,
Forensic,
Ketoprofen,
LC-MS/MS,
Metoprolol,
Mianserin,
Naltrexone,
Neutral,
Pharma,
Pharmaceuticals,
Plasma,
Posters,
Procainamide,
Quinidine,
Ranitidine,
Salbutamol,
Sulindac,
p-toluamide
Evaluation of a Novel 96-well Filter Plate for the Effective Removal of Serum Proteins and Phospholipids prior to LC-MS/MS Analysis
This poster evaluates the performance of a novel 96-well filter plate (ISOLUTE PLD+ Protein and Phospholipid Removal Plate) for
the simultaneous removal of proteins and phospholipids from serum samples prior to LC-MS/MS analysis.
ASMS2014
Tags: 96-well plate,
Acidic,
Amitriptyline,
Analgesics,
Atenolol,
Basic,
Bretylium,
Clinical,
English,
Fluoxetine,
Forensic,
Ketoprofen,
LC-MS/MS,
Metoprolol,
Mianserin,
NSAIDs,
Naltrexone,
Pharma,
Plasma,
Posters,
Procainamide,
Quinidine,
Ranitidine,
Salbutamol,
Serum,
Sulindac,
Tricyclic Antidepressant,
β-blockers
Evaluation of a Novel 96-well Filter Plate for the Effective Removal of Serum Proteins and Phospholipids prior to LC-MS/MS Analysis
The new ISOLUTE® PLD+ Protein and Phospholipid Removal Plate is highlighted in this poster. Protein and phospholipid removal, and analyte recovery are included, along with data illustrating the positive impact of clean (PL and protein free) samples in maintaining analyte signal intensity and low UPLC column back pressure over multiple LCMS runs.
HPLC 2014
Tags: 96-well plate,
Clinical,
English,
Forensic,
LC-MS/MS,
Pharma,
Posters
» PRODUCT NOTES
How to Use ISOLUTE® PLD+ Protein and Phospholipid Depletion Columns
User instructions:ISOLUTE® PLD+ Protein and Phospholipid Depletion Columns
Tags: Clinical,
Column,
English,
Extrahera,
Forensic,
LC-MS/MS,
Pharma,
Product Notes
How to Use ISOLUTE® PLD+ Protein and Phospholipid Depletion Plates
User Instructions: ISOLUTE® PLD+ Protein and Phospholipid Depletion Plates
Tags: 96-well plate,
Clinical,
English,
Extrahera,
Forensic,
Pharma,
Product Notes
ISOLUTE® PLD+ Protein and Phospholipid Removal Products – Questions and Answers
FAQs: ISOLUTE® PLD+ Protein and Phospholipid Removal Products
Tags: 96-well plate,
Clinical,
Column,
English,
Forensic,
LC-MS/MS,
Pharma,
Product Notes
ISOLUTE® PLD+ Protein and Phospholipid Removal Products
Product Note: ISOLUTE® PLD+ Protein and Phospholipid Removal Products
Tags: 96-well plate,
Clinical,
Column,
English,
Forensic,
LC-MS/MS,
Pharma,
Product Notes
Search all documents »
INSTRUCTION VIDEOS
ISOLUTE® PLD+ Protein and Phospholipid Removal Plates
News
02 February 2016Application Suite for Extraction of Methylmalonic Acid
Five new application notes from Biotage labs provide a suite of methods for effective extraction of methylmalonic acid from serum using modern sample preparation techniques and materials from ...
13 October 2015Automated Extraction of Vitamin D from Serum
A new poster from Biotage outlines extraction of 25-hydroxy vitamin D from serum using ISOLUTE® PLD+ and SLE+ on a Biotage® Extrahera™ automated sample preparation platform. &nb...
17 September 2015Press Release – Improved Sensitivity with the New Protein and Phospholipid Removal Columns
Uppsala, Sweden, 17th September, 2015 – Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, is pleased to announce the ...
Biotage® PRESSURE+
Biotage® PRESSURE+ manifolds deliver positive pressure, parallel processing for 96 well plates, 1 mL 3 mL and 6 mL column formats. The systems utilize a consistent, uniform flow of positive pressure to move both low and high viscosity liquids through SPE plates and columns.
Biotage® VacMaster™ 96
Biotage® VacMaster™-96 sample processing manifold has been designed with the needs of the bioanalytical scientist in mind. This manifold is ideal when processing 96-well SPE, supported liquid extraction or protein precipitation plates either manually or with an automated liquid handling system.
Remove More Phospholipids - Get Better Sensitivity
ISOLUTE® PLD+ plates and columns deliver cleaner extracts than other phospholipid removal products, meaning lower detection limits can be achieved. In the example below, signal-to-noise using ISOLUTE PLD+ was more than double when compared with competitor products (figure 1), leading to an immediate enhancement in sensitivity.
Figure 1. Relative peak intensity, 20 pg/mL amitriptyline in plasma (Blue = ISOLUTE® PLD+, Orange = Competitor O, Red= Competitor P).
Excellent Protein and Phospholipid Removal
More than 99 % of phospholipids are removed as the sample passes through the multifunctional sorbent bed, leaving clean, purified extracts for analysis (figure 2). Removing phospholipids also improves analytical column lifetime, preventing the build-up of phospholipids over multiple injections, and maintaining analyte sensitivity over extended analytical runs.
Figure 2. Phospholipids in human plasma treated by a) protein precipitation and b) ISOLUTE® PLD+. Lysophospholipids and phospholipids present in plasma treated by protein precipitation (red and green respectively) are shown in 1b on an expanded scale for direct comparison with residual levels of lysophospholipids and phospholipids present in ISOLUTE® PLD+ treated plasma (purple and blue respectively).
Effective Sample Clean-up in 3 Simple Steps
Figure 3. Typical ISOLUTE PLD+ procedure
Using a simple solvent crash/filtration based procedure, proteins and phospholipids are simultaneously removed from plasma and other blood based samples while high, reproducible analyte recoveries are maintained. The optimised frit arrangement acts as a depth filter, efficiently trapping precipitated proteins, without blocking or plugging.
How to Use ISOLUTE® PLD+ Protein and Phospholipid Depletion Plates
How to Use ISOLUTE® PLD+ Protein and Phospholipid Depletion Columns
Trouble Free Processing
ISOLUTE® PLD+ plates and 'tabless' columns have excellent flow characteristics, and do not plug or block during use. They can be processed easily using either positive pressure or vacuum based systems, and are compatible with most automated liquid handlers.
Up to 96 x 1 mL volume tabless format columns can be processed on Biotage® Pressure+ 96, Extrahera and VacMaster-96 systems using specially designed racks. This cost effective option is ideal for applications with variable sample numbers or for method development.
Find out more about ISOLUTE PLD+ products, along with handy tips for improving your results with our FAQs
CONSUMABLES
| Part No. | Description | Product type | Pack Size | Price | ||
|
121-5202 | Collection Plate, 1 mL Square | Consumables | 50 | Log in for price | |
|
121-5203 | Collection Plate, 2 mL Square | Consumables | 50 | Log in for price | |
|
121-5204 | Pierceable Sealing Cap | Consumables | 50 | Log in for price | |
|
121-5213 | Collection Plate, 2 mL Round | Consumables | 50 | Log in for price | |
|
|
918-0005-AG | ISOLUTE® PLD+ Columns, 50 mg/1 mL (Tabless) | Consumables | 100 | Log in for price | |
|
918-0050-P01 | ISOLUTE® PLD+ Protein and Phospholipid Removal Plate | Consumables | 1 | Log in for price | |